WB
Western blot analysis of beta Actin on different lysates with Rabbit anti-beta Actin antibody at 1/20,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HepG2 cell lysate, Lane 3: MCF7 cell lysate, Lane 4: A431 cell lysate, Lane 5: Jurkat cell lysate, Lane 6: HEK-293 cell lysate, Lane 7: RAW264.7 cell lysate, Lane 8: C2C12 cell lysate, Lane 9: PC-12 cell lysate, Lane 10: Mouse testis tissue lysate, Lane 11: Mouse spleen tissue lysate, Lane 12: Rat testis tissue lysate, Lane 13: Rat spleen tissue lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.| Product Name | HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | le structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia. |
| Immunogen | Synthetic peptide within N-terminal residues of β-Actin. |
| Conjugation | HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:20000-1:50000 |
|
| Species Reactivity | Arabidopsis ThalianaHamsterHumanMonkeyMouseRatRiceZebrafish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | A26C1A antibody
A26C1B antibody
ACTB antibody
ACTB_HUMAN antibody
Actin beta antibody
Actin cytoplasmic 1 antibody
Actin cytoplasmic 1 N-terminally processed antibody Actx antibody b actin antibody Beta cytoskeletal actin antibody Beta-actin antibody BRWS1 antibody E430023M04Rik antibody MGC128179 antibody PS1TP5 binding protein 1 antibody PS1TP5BP1 antibody |
|---|---|
| Molecular Weight(MW) | 42kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of beta Actin on different lysates with Rabbit anti-beta Actin antibody at 1/20,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HepG2 cell lysate, Lane 3: MCF7 cell lysate, Lane 4: A431 cell lysate, Lane 5: Jurkat cell lysate, Lane 6: HEK-293 cell lysate, Lane 7: RAW264.7 cell lysate, Lane 8: C2C12 cell lysate, Lane 9: PC-12 cell lysate, Lane 10: Mouse testis tissue lysate, Lane 11: Mouse spleen tissue lysate, Lane 12: Rat testis tissue lysate, Lane 13: Rat spleen tissue lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.| Application Notes | WB:1:20000-1:50000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
