Application
Fig1: Western blot analysis of FOXA1 on different lysates using anti-FOXA1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293 Lane 2: Mouse lungApplication
Fig2: ICC staining FOXA1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat prostate tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.| Product Name | FOXA1 [JF10-02] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | forkhead box A1 antibody Forkhead box protein A1 antibody FOX A1 antibody FOXA1 antibody FOXA1_HUMAN antibody hepatocyte nuclear factor 3 alpha antibody Hepatocyte nuclear factor 3-alpha antibody HNF 3A antibody HNF-3-alpha antibody HNF-3A antibody HNF3A antibody MGC33105 antibody TCF 3A antibody TCF-3A antibody TCF3A antibody Transcription factor 3A antibody |
| Product description | HNF-1 (α and β), HNF-3 (α, β and γ), HNF-4 (α and γ), and HNF-6 compose, in part, a homoeprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF-3α, HNF-3β and HNF-3γ regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3α and HNF-3β have also been shown to be involved in gastrulation events such as body axis formation. HNF-4α and HNF-4γ have been shown to be important for early embryo development. HNF-4α is expressed in liver, kidney, pancreas, small intestine, testis and colon; HNF-4γ is expressed in each of these tissues except liver. HNF-6 has been shown to bind to the promoter of HNF-3β, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and α-1 antitrypsin. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | forkhead box A1 antibody Forkhead box protein A1 antibody FOX A1 antibody FOXA1 antibody FOXA1_HUMAN antibody hepatocyte nuclear factor 3 alpha antibody Hepatocyte nuclear factor 3-alpha antibody HNF 3A antibody HNF-3-alpha antibody HNF-3A antibody HNF3A antibody MGC33105 antibody TCF 3A antibody TCF-3A antibody TCF3A antibody Transcription factor 3A antibody |
|---|---|
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of FOXA1 on different lysates using anti-FOXA1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293 Lane 2: Mouse lung
Application
Fig2: ICC staining FOXA1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat prostate tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.| Positive Control | 293, MCF-7, rat prostate tissue, human breast tissue, mouse lung tissue, human breast carcinoma tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
