WB
Western blot analysis of Aryl hydrocarbon Receptor on different lysates with Rabbit anti-Aryl hydrocarbon Receptor antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate, Lane 2: A549 cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 2 minutes 37 seconds, 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Aryl hydrocarbon Receptor on different lysates with Rabbit anti-Aryl hydrocarbon Receptor antibody at 1/1,000 dilution. Lane 1: A549-si NT cell lysate, Lane 2: A549-si Aryl hydrocarbon Receptor cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Aryl hydrocarbon Receptor Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is the prototype for a family of toxic halogenated aromatic compounds that are thought to cause adverse reproductive, immunologic and metabolic effects. Many biological responses to TCDD are mediated through ligand binding to the aromatic hydrocarbon (Ah) receptor, also known as AhR. Ah Receptor is a ligand dependent transcription factor that interacts with specific DNA sequences, termed xenobiotic responsive elements (XREs), and that lies upstream of TCDD-inducible genes. Upon binding to the ligand, Ah Receptor binds to the Ah Receptor nuclear translocator (Arnt), and the complex is translocated from the cytoplasm to the nucleus. Arnt is required for Ah R eceptor to bind to XRE. Ah Receptor and Arnt are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common "PAS" motif. |
| Immunogen | Synthetic peptide within Human Aryl hydrocarbon Receptor aa 735-775 / 848. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Ah receptor antibody AhR antibody AHR_HUMAN antibody Aromatic hydrocarbon receptor antibody Aryl hydrocarbon receptor antibody Aryl hydrocarbon receptor precursor antibody bHLHe76 antibody Class E basic helix loop helix protein 76 antibody Class E basic helix-loop-helix protein 76 antibody HGNC:348 antibody |
|---|---|
| Molecular Weight(MW) | 96 kDa(Observed band size: 105 kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | P35869 |
|---|
WB
Western blot analysis of Aryl hydrocarbon Receptor on different lysates with Rabbit anti-Aryl hydrocarbon Receptor antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate, Lane 2: A549 cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 2 minutes 37 seconds, 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Aryl hydrocarbon Receptor on different lysates with Rabbit anti-Aryl hydrocarbon Receptor antibody at 1/1,000 dilution. Lane 1: A549-si NT cell lysate, Lane 2: A549-si Aryl hydrocarbon Receptor cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
