WB
Western blot analysis of GST on different lysates with Rabbit anti-GST antibody at 1/5,000 dilution. Lane 1: 293T transfected with GST cell lysate (5 µg/Lane), Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate (15 µg/Lane), Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.| Product Name | HRP Conjugated GST Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. Mammalian cytosolic GSTs are dimeric both subunits being from the same class of GSTs. The monomers are in the range of 22–29 kDa. Glutathione S-transferase is used to create the “GST gene fusion system” in genetic engineering. Here, GST is used to purify and detect proteins of interest. |
| Immunogen | recombinant protein |
| Conjugation | HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:1,000-1:5,000 |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Glutathione S Transferase antibody Glutathione S-transferase class-mu 26 kDa isozyme antibody GST antibody |
|---|
WB
Western blot analysis of GST on different lysates with Rabbit anti-GST antibody at 1/5,000 dilution. Lane 1: 293T transfected with GST cell lysate (5 µg/Lane), Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate (15 µg/Lane), Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.| Positive Control | GST protein. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
