Application
Fig1: Flow cytometric analysis of Hela cells with NOTCH4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | NOTCH4 [JM101-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ16302 antibody hNotch 4 antibody hNotch4 antibody INT3 antibody Neurogenic locus notch homolog protein 4 antibody NOTC4_HUMAN antibody Notch 4 antibody Notch 4 intracellular domain antibody Notch homolog 4 antibody NOTCH3 antibody Notch4 antibody NOTCH4 protein antibody |
| Product description | The LIN-12/Notch transmembrane receptors are believed to play a central role in development by regulating cell fate decisions. Four Notch homologs (Notch 1, Notch 2, Notch 3 and Notch 4) have been identified in mammals. The Notch genes are expressed in a variety of embryonic and adult tissues, suggesting that the genes are involved in multiple signaling pathways. Notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for Notch include Jagged1, Jagged2 and Delta. Jagged1 can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. Jagged2 may be involved in tissue development that is dependent upon epithelial-mesenchymal interactions. In addition to its normal expression in the adrenal gland and placenta, Delta expression has also been found in neuroendocrine tumors. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBFC |
| WB:1:500-1:1,000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | FLJ16302 antibody hNotch 4 antibody hNotch4 antibody INT3 antibody Neurogenic locus notch homolog protein 4 antibody NOTC4_HUMAN antibody Notch 4 antibody Notch 4 intracellular domain antibody Notch homolog 4 antibody NOTCH3 antibody Notch4 antibody NOTCH4 protein antibody |
|---|---|
| Molecular Weight(MW) | 210 kDa |
| Cellular Localization | Cell membrane, Nucleus. |
Application
Fig1: Flow cytometric analysis of Hela cells with NOTCH4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela, human kdiney tissue, mouse lung tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
