Application
Fig1: Western blot analysis of CD40L on different cells lysates using anti-CD40L antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: Mouse liverApplication
Fig2: ICC staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | CD40L [JM11-34] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 40L antibody CD154 antibody CD40 antigen ligand antibody CD40 ligand antibody CD40 ligand, soluble form antibody CD40-L antibody CD40L antibody CD40L_HUMAN antibody CD40LG antibody gp39 antibody hCD40L antibody HIGM1 antibody IGM antibody IMD3 antibody T B cell activating molecule antibody T BAM antibody T-cell antigen Gp39 antibody TNF-related activation protein antibody TNFSF5 antibody TrAP antibody Tumor necrosis factor (ligand) superfamily member 5 antibody Tumor necrosis factor ligand superfamily member 5 antibody |
| Product description | Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. While cytokines IL-4 and IL-13 alone are inadequate for B cell activation, contact with Th cells seems to be sufficient for delivery of proliferative signals. CD40 and CD154 (also designated CD40L or TRAP) comprise a receptor ligand pair central to the transmission of this signal. CD40 is expressed on the surface of B cells and CD154 is expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. CD154 is a 261 amino acid protein that is is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. Its expression is tightly regulated, and abnormal levels of CD154 are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | CD 40L antibody
CD154 antibody
CD40 antigen ligand antibody
CD40 ligand antibody
CD40 ligand soluble form antibody CD40-L antibody CD40L antibody CD40L_HUMAN antibody CD40LG antibody gp39 antibody hCD40L antibody HIGM1 antibody IGM antibody IMD3 antibody T B cell activating molecule antibody T BAM antibody T-cell antigen Gp39 antibody TNF-related activation protein antibody TNFSF5 antibody TrAP antibody Tumor necrosis factor (ligand) superfamily member 5 antibody Tumor necrosis factor ligand superfamily member 5 antibody |
|---|---|
| Molecular Weight(MW) | 36 kDa |
| Cellular Localization | Cell membrane, Cell surface, Secreted. |
Application
Fig1: Western blot analysis of CD40L on different cells lysates using anti-CD40L antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: Mouse liver
Application
Fig2: ICC staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela, MCF-7, NIH-3T3, THP-1, mouse liver cells lysates, human spleen tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
