WB
Western blot analysis of GAD67 on different lysates with Rabbit anti-GAD67 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: SH-SY5Y cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | GAD67 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD-65 (also known as GAD2) and GAD-67 (also known as GAD1, GAD or SCP). GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (g-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67, the predominant GAD-67 form and the minor GAD-25 form. GAD-25 is not expressed in brain but can be found in a variety of endocrine tissues. |
| Immunogen | Synthetic peptide within Human GAD67 aa 60-99 / 594. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:500-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | 67 kDa glutamic acid decarboxylase antibody
CPSQ1 antibody
DCE1 antibody
DCE1_HUMAN antibody
EC 4.1.1.15 antibody
FLJ45882 antibody
GAD 67 antibody
GAD antibody
GAD-67 antibody
GAD1 antibody
Glutamate decarboxylase 1 (brain 67kDa) antibody Glutamate decarboxylase 1 antibody Glutamate decarboxylase 1 brain 67kD antibody Glutamate decarboxylase 1 brain 67kDa antibody Glutamate decarboxylase 67 kDa isoform antibody Glutamate decarboxylase brain 67-KD antibody OTTHUMP00000041055 antibody SCP antibody |
|---|---|
| Molecular Weight(MW) | 67 kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of GAD67 on different lysates with Rabbit anti-GAD67 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: SH-SY5Y cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
