Application
Fig1: ICC staining NFAT1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining NFAT1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining NFAT1 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NFAT1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NFAT1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NFAT1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Jurkat cells with NFAT1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | NFAT1 [JA11-08] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AI607462 antibody cytoplasmic 2 antibody KIAA0611 antibody NF ATc2 antibody NF ATp antibody NF-ATc2 antibody NF-ATp antibody NFAC2_HUMAN antibody NFAT 1 antibody NFAT pre existing subunit antibody NFAT pre-existing subunit antibody NFAT transcription complex, preexisting component antibody NFAT1 antibody NFAT1-D antibody NFATc2 antibody NFATp antibody Nuclear factor of activated T cells cytoplasmic 2 antibody Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody Nuclear factor of activated T cells pre-existing component antibody Nuclear factor of activated T cells, preexisting component antibody Nuclear factor of activated T-cells antibody Preexisting nuclear factor of activated T cells 2 antibody T cell transcription factor NFAT 1 antibody T-cell transcription factor NFAT1 antibody |
| Product description | The NFAT (nuclear factor of activated T cells) family of transcription factors regulates cytokine expression in T cells. Members of the family include NFATc1 (NFATc), NFATc2 (NFATp), NFATn, NFATc3 (NFAT4, NFATx) and NFATc4 (NFAT3). Recognition of antigen by the T cell receptor (TCR) eventually activates the calcium-dependent protein phosphatase calcineurin. Once activated, calcineurin stimulates the translocation of NFATc1 (cytoplasmic) from the cytoplasm to the nucleus where it associates with NFATn (nuclear). Like NFATc1, NFATc2 resides in the cytoplasm and translocates to the nucleus subsequent to activation of calcineurin. Once in the nucleus, NFATc2 synergizes with AP-1 transcription factors to initiate transcription of cytokine genes. NFATc3 and NFATc4 share 65% sequence identity with other members of the NFAT family. They are similar to NFATc2 in that they also synergize with the AP-1 family of proteins. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | AI607462 antibody
cytoplasmic 2 antibody
KIAA0611 antibody
NF ATc2 antibody
NF ATp antibody
NF-ATc2 antibody
NF-ATp antibody
NFAC2_HUMAN antibody
NFAT 1 antibody
NFAT pre existing subunit antibody
NFAT pre-existing subunit antibody
NFAT transcription complex preexisting component antibody NFAT1 antibody NFAT1-D antibody NFATc2 antibody NFATp antibody Nuclear factor of activated T cells cytoplasmic 2 antibody Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody Nuclear factor of activated T cells pre-existing component antibody Nuclear factor of activated T cells preexisting component antibody Nuclear factor of activated T-cells antibody Preexisting nuclear factor of activated T cells 2 antibody T cell transcription factor NFAT 1 antibody T-cell transcription factor NFAT1 antibody |
|---|---|
| Molecular Weight(MW) | 135 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | Q13469 |
|---|
Application
Fig1: ICC staining NFAT1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining NFAT1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining NFAT1 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NFAT1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NFAT1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NFAT1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Jurkat cells with NFAT1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Jurkat, MCF-7, Hela, SHG-44, human tonsil tissue, human spleen tissue, human colon cancer tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
