Application
Fig1: Western blot analysis of MSH6 on Hela cell lysates using anti-MSH6 at 1/500 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-MSH6 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MSH6 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-MSH6 antibody. Counter stained with hematoxylin.| Product Name | MSH6 [JA84-26] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA mismatch repair protein Msh6 antibody G/T mismatch binding protein antibody G/T mismatch-binding protein antibody GTBP antibody GTMBP antibody hMSH6 antibody HNPCC 5 antibody HNPCC5 antibody HSAP antibody MSH 6 antibody MSH6 antibody MSH6_HUMAN antibody mutS (E. coli) homolog 6 antibody MutS alpha 160 kDa subunit antibody MutS homolog 6 (E. coli) antibody mutS homolog 6 antibody MutS-alpha 160 kDa subunit antibody p160 antibody Sperm associated protein antibody |
| Product description | Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP>ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500-1:2,000 IHC:1:50-1:200 IP:1:10-1:50 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | DNA mismatch repair protein Msh6 antibody G/T mismatch binding protein antibody G/T mismatch-binding protein antibody GTBP antibody GTMBP antibody hMSH6 antibody HNPCC 5 antibody HNPCC5 antibody HSAP antibody MSH 6 antibody MSH6 antibody MSH6_HUMAN antibody mutS (E. coli) homolog 6 antibody MutS alpha 160 kDa subunit antibody MutS homolog 6 (E. coli) antibody mutS homolog 6 antibody MutS-alpha 160 kDa subunit antibody p160 antibody Sperm associated protein antibody |
|---|---|
| Molecular Weight(MW) | 153 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of MSH6 on Hela cell lysates using anti-MSH6 at 1/500 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-MSH6 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MSH6 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-MSH6 antibody. Counter stained with hematoxylin.| Positive Control | Hela, human tonsil tissue, mouse liver tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
