WB
Fig1: Western blot analysis of Integrin alpha 4 on different lysates with Rabbit anti-Integrin alpha 4 antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: Mouse spleen tissue lysate (40 µg/Lane) Lane 3: Rat spleen tissue lysate (40 µg/Lane) Exposure time: Lane 1: 25 seconds; Lane 2-3: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Integrin alpha 4 antibody. Counter stained with hematoxylin.Application
Fig3: Flow cytometric analysis of Jurkat cells with Integrin alpha 4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.| Product Name | Integrin alpha 4 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Integrins are heterodimers composed of noncovalently associated transmembrane α and β subunits. The 16 α and 8 β subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including fibronectin, collagen and vitronectin. Certain integrins can also bind to soluble ligands such as fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. |
| Immunogen | Synthetic peptide within Human Integrin alpha 4 aa 983-1032 / 1032. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCIHCWB |
| WB:1:1000 IHC:1:50-1:200 FC:1:10-1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | 269C wild type antibody
Antigen CD49D alpha 4 subunit of VLA 4 receptor antibody CD49 antigen like family member D antibody CD49 antigen-like family member D antibody CD49d antibody IA4 antibody Integrin alpha 4 antibody Integrin alpha 4 subunit antibody Integrin alpha IV antibody Integrin alpha-4 antibody Integrin alpha-IV antibody Integrin alpha 4 (antigen CD49D alpha 4 subunit of VLA 4 receptor) antibody ITA4_HUMAN antibody ITGA4 antibody MGC90518 antibody OTTHUMP00000205320 antibody Very late activation protein 4 receptor alpha 4 subunit antibody VLA 4 subunit alpha antibody VLA-4 subunit alpha antibody VLA4 antibody |
|---|---|
| Molecular Weight(MW) | 150 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P13473 |
|---|
WB
Fig1: Western blot analysis of Integrin alpha 4 on different lysates with Rabbit anti-Integrin alpha 4 antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: Mouse spleen tissue lysate (40 µg/Lane) Lane 3: Rat spleen tissue lysate (40 µg/Lane) Exposure time: Lane 1: 25 seconds; Lane 2-3: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Integrin alpha 4 antibody. Counter stained with hematoxylin.
Application
Fig3: Flow cytometric analysis of Jurkat cells with Integrin alpha 4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.| Positive Control | Jurkat, human tonsil tissue, human spleen tissue. |
|---|---|
| Application Notes | WB:1:1000 IHC:1:50-1:200 FC:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
