Application
Fig1: Western blot analysis of Profilin 1 on Hela cell using anti-Profilin 1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining Profilin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Profilin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Profilin 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of Hela cells with Profilin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | Profilin 1 [JA30-13] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Actin binding protein antibody ALS18 antibody Epididymis tissue protein Li 184a antibody OTTHUMP00000125244 antibody PFN 1 antibody Pfn antibody PFN1 antibody PROF1_HUMAN antibody Profilin I antibody Profilin-1 antibody Profilin1 antibody ProfilinI antibody |
| Product description | Profilins regulate Actin polymerization by binding to and sequestering the Actin monomer. Profilins act as a nucleotide exchange factor that charges Actin with ATP after binding the Actin monomer through a 1:1 stoichiometric relationship. Human Profilin-1 and Profilin-2 are encoded by two separate genes mapping to chromosomes 17p13.2 and 3q25.1, respectively. Both Profilin-1 and Profilin-2 are abundantly expressed in kidney. Profilin-1 is highly expressed in lung, liver, placenta and kidney while Profilin-2 is highly expressed in brain and skeletal muscle. In axonal and dendritic processes of mouse brain, Profilins co-localize with dynamin I and synapsin. Profilin may play a role in mediating cell adhesion. The overexpression of Profilin in endothelial cells results in increased adhesion to Fibronectin. In food allergy, plant Profilin is considered a pan-allergen. Case studies indicate individuals with allergies to various foods including celery, carrots, zucchini and peanuts are actually sensitive to the Profilin proteins in these foods. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Actin binding protein antibody ALS18 antibody Epididymis tissue protein Li 184a antibody OTTHUMP00000125244 antibody PFN 1 antibody Pfn antibody PFN1 antibody PROF1_HUMAN antibody Profilin I antibody Profilin-1 antibody Profilin1 antibody ProfilinI antibody |
|---|---|
| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Cytoplasm, cytoskeleton. |
Application
Fig1: Western blot analysis of Profilin 1 on Hela cell using anti-Profilin 1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Profilin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Profilin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Profilin 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Hela cells with Profilin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | HepG2, Hela, A549, human kidney tissue, mouse colon tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
