Application
Fig1: Western blot analysis of CD163 on human thymus tissue lysate using anti-CD163 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD163 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD163 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD163 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of THP-1 cells with CD163 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | CD163 [JA51-30] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C163A_HUMAN antibody CD 163 antibody CD163 antibody CD163 antigen antibody CD163 molecule antibody Hemoglobin scavenger receptor antibody M130 antibody M130 antigen precursor antibody Macrophage associated antigen antibody MM130 antibody OTTHUMP00000238617 antibody OTTHUMP00000238618 antibody OTTHUMP00000238619 antibody OTTHUMP00000238620 antibody SCARI1 antibody Scavenger receptor cysteine rich type 1 protein M130 antibody sCD163 antibody Soluble CD163 antibody |
| Product description | CD163, also designated M130, is a macrophage-associated antigen that is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. It is highly expressed on macrogphages and to a lesser extent on monocytes. The acute phase-regulated and signal-inducing macrophage protein, CD163, is a receptor that scavenges hemoglobin by mediating endocytosis of haptoglobin-hemoglobin complexes. CD163 binds only haptoglobin and hemoglobin in complex, which indicates the exposure of a receptor-binding neoepitope. The receptor-ligand interaction is calcium-dependent and of high affinity. The existence of several CD163 isoforms, which differ in the structure of their cytoplasmic domains and putative phosphorylation sites, suggests that these isoforms also differ in their signaling mechanism. The gene which encodes CD163 maps to human chromosome 12p13.31. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIPFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | C163A_HUMAN antibody CD 163 antibody CD163 antibody CD163 antigen antibody CD163 molecule antibody Hemoglobin scavenger receptor antibody M130 antibody M130 antigen precursor antibody Macrophage associated antigen antibody MM130 antibody OTTHUMP00000238617 antibody OTTHUMP00000238618 antibody OTTHUMP00000238619 antibody OTTHUMP00000238620 antibody SCARI1 antibody Scavenger receptor cysteine rich type 1 protein M130 antibody sCD163 antibody Soluble CD163 antibody |
|---|---|
| Molecular Weight(MW) | 150 kDa |
| Cellular Localization | Secreted and Cell membrane. |
| SwissProt ID | Q86VB7 |
|---|
Application
Fig1: Western blot analysis of CD163 on human thymus tissue lysate using anti-CD163 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD163 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD163 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD163 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of THP-1 cells with CD163 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | Human thymus tissue lysate, human liver tissue, human spleen tissue, human placenta tissue, THP-1. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
