WB
Western blot analysis of Clathrin heavy chain on different lysates with Rabbit anti-Clathrin heavy chain antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: A431 cell lysate, Lane 3: NIH/3T3 cell lysate, Lane 4: PC-12 cell lysate, Lane 5: HUVEC cell lysate, Lane 6: SH-SY5Y cell lysate, Lane 7: COS-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Clathrin heavy chain antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Clathrin heavy chain Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Clathrin is a major cytosolic coat protein in pits and vesicles originating from the plasma membrane and the trans-Golgi network. In receptor-mediated endocytosis, receptor proteins are englufed by clathrin-coated vesicles. Clathrin is composed of three heavy chains and three light chains which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain binding site. The proximal segment of the Clathrin HC protein is essential for interactions between clathrin heavy chains and light chains which result in the formation of the triskelion structure. |
| Immunogen | Synthetic peptide within Human Clathrin heavy chain aa 201-250 / 1,675. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:200 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | CHC17 antibody Clathrin heavy chain antibody Clathrin heavy chain 1 antibody Clathrin heavy chain 2 antibody Clathrin heavy chain like 1 antibody Clathrin heavy chain on chromosome 17 antibody Clathrin heavy polypeptide antibody CLH 17 antibody CLH 22 antibody CLH-17 antibody CLH1_HUMAN antibody CLH17 antibody CLH22 antibody CLTC antibody CLTCL antibody CLTCL1 antibody CLTCL2 antibody CLTD antibody Hc antibody KIAA0034 antibody |
|---|---|
| Molecular Weight(MW) | 180 kDa |
| Cellular Localization | Cytoplasmic vesicle membrane. |
WB
Western blot analysis of Clathrin heavy chain on different lysates with Rabbit anti-Clathrin heavy chain antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: A431 cell lysate, Lane 3: NIH/3T3 cell lysate, Lane 4: PC-12 cell lysate, Lane 5: HUVEC cell lysate, Lane 6: SH-SY5Y cell lysate, Lane 7: COS-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Clathrin heavy chain antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
