WB
Western blot analysis of CDT1 on different lysates with Rabbit anti-CDT1 antibody at 1/2,000 dilution. Lane 1: HCT 116 cell lysate, Lane 2: HT-29 cell lysate, Lane 3: Jurkat cell lysate, Lane 4: HEK-293 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CDT1 antibody. Counter stained with hematoxylin.ICC/IF
Immunocytochemistry analysis of HT-29 cells labeling CDT1 with Rabbit anti-CDT1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDT1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | CDT1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Human Cdt1 is a nuclear localizing replication initiation factor that is expressed only during the G1 and S phases of the cell cycle. In conjunction with Cdc18, Cdt1 is required to load the MCM protein Cdc21 onto chromatin at the end of mitosis which is necessary to initiate DNA replication. After S-phase onset, Cdt1 protein levels decrease and are barely detectable in cells in early S-phase or G2. However, Cdt1 mRNA is expressed in S-phase-arrested cells, and its levels do not change dramatically during the cell cycle, suggesting that proteolytic degradation rather than transcriptional controls ensure proper accumulation of Cdt1. Cdt1 can associate with the DNA replication inhibitor geminin, which is present in the S and G2 phases of the cell cycle. Inhibition of DNA replication by geminin in cell-free DNA replication extracts can be reversed by the addition of excess Cdt1. Geminin may be responsible for preventing inappropriate origin firing by targeting Cdt1. |
| Immunogen | Recombinant protein within Human CDT1 aa 497-546 / 546. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000 IHC:1:25-1:50 ICC/IF:1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | CDT 1 antibody cdt1 antibody CDT1_HUMAN antibody Chromatin licensing and DNA replication factor 1 antibody DNA replication factor antibody DNA replication factor Cdt1 antibody Double parked antibody Double parked Drosophila homolog of antibody Double parked homolog antibody DUP antibody Retroviral integration site 1 antibody Retroviral integration site 2 antibody Retroviral integration site1 antibody Retroviral integration site2 antibody RIS 2 antibody RIS2 antibody |
|---|---|
| Molecular Weight(MW) | 60kDa(Observed band size: 70kDa) |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9H211 |
|---|
WB
Western blot analysis of CDT1 on different lysates with Rabbit anti-CDT1 antibody at 1/2,000 dilution. Lane 1: HCT 116 cell lysate, Lane 2: HT-29 cell lysate, Lane 3: Jurkat cell lysate, Lane 4: HEK-293 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CDT1 antibody. Counter stained with hematoxylin.
ICC/IF
Immunocytochemistry analysis of HT-29 cells labeling CDT1 with Rabbit anti-CDT1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDT1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:2000 IHC:1:25-1:50 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
