Application
Fig1: Western blot analysis of PKC epsilon on different cell lysate using anti-PKC epsilon antibody at 1/1,000 dilution. Positive control： Lane1: Jurkat Lane2: MCF-7 Lane3: HT-29Application
Fig2: ICC staining PKC epsilon in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PKC epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of SH-SY5Y cells with PKC epsilon antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | PKC epsilon [JA38-21] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KPCE_HUMAN antibody MGC125656 antibody MGC125657 antibody nPKC epsilon antibody nPKC-epsilon antibody PKCE antibody Pkcea antibody PRKCE antibody Protein kinase C epsilon antibody Protein kinase C epsilon type antibody |
| Product description | This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | KPCE_HUMAN antibody MGC125656 antibody MGC125657 antibody nPKC epsilon antibody nPKC-epsilon antibody PKCE antibody Pkcea antibody PRKCE antibody Protein kinase C epsilon antibody Protein kinase C epsilon type antibody |
|---|---|
| Molecular Weight(MW) | 84 kDa |
| Cellular Localization | Cytoplasm.?Membrane.?Nucleus. |
Application
Fig1: Western blot analysis of PKC epsilon on different cell lysate using anti-PKC epsilon antibody at 1/1,000 dilution. Positive control： Lane1: Jurkat Lane2: MCF-7 Lane3: HT-29
Application
Fig2: ICC staining PKC epsilon in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PKC epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of SH-SY5Y cells with PKC epsilon antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | Jurkat, MCF-7, HT-29, 293T, Hela, SH-SY5Y, rat brain tissue, human pancreas tissue, mouse testis tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
