Application
Fig1: Western blot analysis of Calpain 2 on Hela cell (1) and rat skin tissue (2) lysate using anti-Calpain 2 antibody at 1/1,000 dilution.Application
Fig2: ICC staining Calpain 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with Calpain 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | Calpain 2 [JA43-41] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Calcium activated neutral proteinase 2 antibody Calcium activated neutral proteinase antibody Calcium-activated neutral protease 2, catalytic subunit antibody Calcium-activated neutral proteinase 2 antibody CALP80 antibody Calpain 2 (m/II) large subunit antibody Calpain 2 catalytic subunit antibody Calpain 2 large catalytic subunit antibody Calpain 2 large subunit antibody Calpain 2, large [catalytic] subunit antibody Calpain large polypeptide L2 antibody Calpain M type antibody Calpain M-type antibody Calpain-2 catalytic subunit antibody Calpain-2 large subunit antibody Calpain2 antibody CAN2_HUMAN antibody CANP 2 antibody CANP L2 antibody CANP2 antibody CANPL 2 antibody CANPL2 antibody CANPml antibody Capa2 antibody CAPN 2 antibody CAPN2 antibody FLJ39928 antibody M calpain antibody M calpin antibody M type antibody M-calpain antibody mCANP antibody Millimolar calpain antibody Millimolar-calpain antibody |
| Product description | Calpain, an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, is thought to play a role in cytoskeletal reorganization and muscle protein degradation. Calpain exists as a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates calpain by inhibiting both the proteolytic activity of calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Calcium activated neutral proteinase 2 antibody
Calcium activated neutral proteinase antibody
Calcium-activated neutral protease 2 catalytic subunit antibody Calcium-activated neutral proteinase 2 antibody CALP80 antibody Calpain 2 (m/II) large subunit antibody Calpain 2 catalytic subunit antibody Calpain 2 large catalytic subunit antibody Calpain 2 large subunit antibody Calpain 2 large [catalytic] subunit antibody Calpain large polypeptide L2 antibody Calpain M type antibody Calpain M-type antibody Calpain-2 catalytic subunit antibody Calpain-2 large subunit antibody Calpain2 antibody CAN2_HUMAN antibody CANP 2 antibody CANP L2 antibody CANP2 antibody CANPL 2 antibody CANPL2 antibody CANPml antibody Capa2 antibody CAPN 2 antibody CAPN2 antibody FLJ39928 antibody M calpain antibody M calpin antibody M type antibody M-calpain antibody mCANP antibody Millimolar calpain antibody Millimolar-calpain antibody |
|---|---|
| Molecular Weight(MW) | 80 kDa |
| Cellular Localization | Cytoplasm. Cell membrane. |
Application
Fig1: Western blot analysis of Calpain 2 on Hela cell (1) and rat skin tissue (2) lysate using anti-Calpain 2 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Calpain 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with Calpain 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | Hela, rat skin tissue lysate, human lung tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
