WB
Western blot analysis of Peroxiredoxin 3 on different lysates with Rabbit anti-Peroxiredoxin 3 antibody at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.ICC/IF
ICC staining Peroxiredoxin 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.ICC/IF
ICC staining Peroxiredoxin 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.ICC/IF
ICC staining Peroxiredoxin 3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.FC
Flow cytometric analysis of MCF-7 cells with Peroxiredoxin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | Peroxiredoxin 3 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis. The human PRX IV gene is abundantly expressed in many tissues. |
| Immunogen | Synthetic peptide within Human Peroxiredoxin 3 aa 207-256 / 256. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Antioxidant protein 1 antibody AOP 1 antibody AOP-1 antibody AOP1 antibody HBC189 antibody MER5 antibody MGC104387 antibody MGC24293 antibody mitochondrial antibody peroxiredoxin 3 antibody Peroxiredoxin III antibody Peroxiredoxin-3 antibody PRDX3 antibody PRDX3_HUMAN antibody PRO1748 antibody Protein MER5 homolog antibody PRX III antibody Prx-III antibody PRX3 antibody SP 22 antibody SP-22 antibody SP22 antibody Thioredoxin dependent peroxide reductase mitochondrial antibody Thioredoxin-dependent peroxide reductase antibody |
|---|---|
| Molecular Weight(MW) | 20 kDa |
| Cellular Localization | Mitochondrion. |
| SwissProt ID | P30048 |
|---|
WB
Western blot analysis of Peroxiredoxin 3 on different lysates with Rabbit anti-Peroxiredoxin 3 antibody at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
ICC/IF
ICC staining Peroxiredoxin 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC/IF
ICC staining Peroxiredoxin 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC/IF
ICC staining Peroxiredoxin 3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.
FC
Flow cytometric analysis of MCF-7 cells with Peroxiredoxin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
