WB
Western blot analysis of C4 on human liver tissue lysate using anti-C4 antibody at 1/1,000 dilution.ICC/IF
ICC staining C4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.IHC
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-C4 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | C4 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The complement component proteins C3, C4 and C5 are potent anaphylatoxins that are released during complement activation, a system of ligand-surface protein interactions specific to cells of hematopoietic lineage. These proteins belong to the α2-macroglobulin family, but retain distinctive features including an anaphylatoxin domain and a netrin (NTR) domain. The human C4 gene is polymorphic at two loci, C4A and C4B, mapping to chromosome 6p21.32. C4A expresses the Rodgers (Rg) blood group Ag, while C4B expresses the Chido (Ch) blood group Ag. C4 is expressed as a precursor that is cleaved into α, β and γ chains, all of which are non-identical cleavage products. The α chain of C4 may be cleaved to produce an acidic isotype, C4a, which reacts with amino groups, and a basic isotype, C4b, which reacts with hydroxyl groups. Deficiency in the C4 gene is associated with autoimmune or immune complex disorders, such as systemic lupus erythematosus. |
| Immunogen | Synthetic peptide within Human C4 aa 1,231-1,280 / 1,744. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Acidic C4 antibody
Acidic complement C4 antibody Basic C4 antibody Basic complement C4 antibody C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody C4 Chido form antibody C4 Rodgers from antibody C4A anaphylatoxin antibody C4A antibody C4A2 antibody C4A3 antibody C4A4 antibody C4A6 antibody C4AD antibody C4B antibody C4B_2 antibody C4B1 antibody C4B12 antibody C4B2 antibody C4B3 antibody C4BD antibody C4F antibody C4S antibody CH antibody Chido form of C4 antibody CO4 antibody CO4A_HUMAN antibody Complement C4 gamma chain antibody complement C4-A antibody complement C4-B antibody complement C4-B-like antibody complement C4B1a antibody Complement component 4A (Rodgers blood group) antibody Complement component 4A antibody complement component 4B (Chido blood group) copy 2 antibody Complement component 4B (Childo blood group) antibody Complement component 4B antibody Complement component 4F antibody Complement component 4S antibody CPAMD2 antibody CPAMD3 antibody RG antibody Rodgers form of C4 antibody |
|---|---|
| Molecular Weight(MW) | 193 kDa |
| Cellular Localization | Cell junction, Cell projection, Secreted, Synapse. |
| SwissProt ID | P0C0L4 |
|---|
WB
Western blot analysis of C4 on human liver tissue lysate using anti-C4 antibody at 1/1,000 dilution.
ICC/IF
ICC staining C4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
IHC
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-C4 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
