Application
Fig1: Western blot analysis of MUC4 on PC-3M cell using anti-MUC4 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MUC4 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MUC4 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MUC4 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-MUC4 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of A549 cells with MUC4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | MUC4 [JM19-36] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ascites sialoglycoprotein 1 antibody Ascites sialoglycoprotein 2 antibody Ascites sialoglycoprotein antibody ASGP antibody ASGP-1 antibody ASGP-2 antibody HSA276359 antibody MUC 4 antibody MUC-4 antibody Muc4 antibody MUC4_HUMAN antibody Mucin 4 antibody Mucin 4 cell surface associated antibody Mucin 4 tracheobronchial antibody Mucin-4 beta chain antibody Pancreatic adenocarcinoma mucin antibody Testis mucin antibody Tracheobronchial mucin antibody Tracheobronchial mucin Fragment antibody |
| Product description | Mucins are a group of high molecular weight glycoproteins consisting of a mucin core protein and O-linked carbohydrates. Mucin 4, a membrane-bound mucin, is the human homolog of the rat sialomucin complex (SMC). Mucin 4 protein consists of Mucin 4α, a large amino mucin type subunit, and Mucin 4β, a transmembrane subunit containing three EGF-like domains. The Mucin 4 gene is the predominant mucin gene expressed in the normal urothelium and is also expressed in several normal tissues such as trachea, lung and testis. Dysregulation of Mucin 4 results in high levels of expression in pancreatic tumors and tumor cell lines. Induction of Mucin 4 in pancreatic carcinoma by all-trans-retinoic acid is mediated through the retinoic acid receptor-α signaling pathway. TGFβ2 serves as an interim mediator of this regulated expression. Alternative splicing in the 3'-end of the Mucin 4 gene generates at least 12 splice variants, which are characterized as two distinct types, a secreted type and a membrane-associated type. Mucin 4 protein acts as a heterodimeric bifunctional cell-surface glycoprotein and forms thick mucous effusion in the diseased middle ear. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFC |
| WB:1:500~1:2000 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | Ascites sialoglycoprotein 1 antibody Ascites sialoglycoprotein 2 antibody Ascites sialoglycoprotein antibody ASGP antibody ASGP-1 antibody ASGP-2 antibody HSA276359 antibody MUC 4 antibody MUC-4 antibody Muc4 antibody MUC4_HUMAN antibody Mucin 4 antibody Mucin 4 cell surface associated antibody Mucin 4 tracheobronchial antibody Mucin-4 beta chain antibody Pancreatic adenocarcinoma mucin antibody Testis mucin antibody Tracheobronchial mucin antibody Tracheobronchial mucin Fragment antibody |
|---|---|
| Molecular Weight(MW) | 120 kDa |
| Cellular Localization | Secreted, Cell membrane and Membrane. |
| SwissProt ID | Q99102 |
|---|
Application
Fig1: Western blot analysis of MUC4 on PC-3M cell using anti-MUC4 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MUC4 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MUC4 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MUC4 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-MUC4 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of A549 cells with MUC4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | PC-3M cell lysate, rat brain tissue, human lung caner tissue, human colon tissue, human stomach cancer tissue, A549. |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
