Application
Fig1: Western blot analysis of TAK1 on human placenta tissue lysate using anti-TAK1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining TAK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining TAK1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-TAK1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-TAK1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-TAK1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of A431 cells with TAK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | TAK1 [JM73-19] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | M3K7_HUMAN antibody MAP3K 7 antibody Map3k7 antibody MEKK7 antibody Mitogen activated protein kinase kinase kinase 7 antibody Mitogen-activated protein kinase kinase kinase 7 antibody TAK1 antibody TGF beta activated kinase 1 antibody TGF-beta-activated kinase 1 antibody TGF1a antibody Transforming growth factor beta activated kinase 1 antibody Transforming growth factor-beta-activated kinase 1 antibody |
| Product description | ediates in signal transduction pathways. These include ERK / MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a protein with intrinsic kinase activity towards serine / threonine residues and Which is expressed expression in many human types and cell lines. Raf-1 activation is dependent on the small molecular weight GTPase Ras, but the means by which this activation is poorly understood. Two proteins put put involved in this process are Ksr-1 and Taks. Ksr-1 (kinase sup-pressor of Ras) is a novel Raf-related protein kinase whose function is required for Ras signal transduction. Whether or not in a parallel pathway is not yet known. Tak1 (TGFβ-activated kinase) has been shown to participate in the activation of the MAP kinase family in response to TGFβ stimulation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:500~1:2000 ICC/IF:1:200~1:500 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | M3K7_HUMAN antibody MAP3K 7 antibody Map3k7 antibody MEKK7 antibody Mitogen activated protein kinase kinase kinase 7 antibody Mitogen-activated protein kinase kinase kinase 7 antibody TAK1 antibody TGF beta activated kinase 1 antibody TGF-beta-activated kinase 1 antibody TGF1a antibody Transforming growth factor beta activated kinase 1 antibody Transforming growth factor-beta-activated kinase 1 antibody |
|---|---|
| Molecular Weight(MW) | 67 kDa |
| Cellular Localization | Cell membrane. Cytoplasm. |
Application
Fig1: Western blot analysis of TAK1 on human placenta tissue lysate using anti-TAK1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining TAK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining TAK1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-TAK1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-TAK1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-TAK1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of A431 cells with TAK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | Human placenta tissue lysate, A431, N2A, human prostate tissue, human placenta tissue, mouse stomach tissue. |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC/IF:1:200~1:500 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
