Application
Fig1: Western blot analysis of PKC beta 1 on L929 cell using anti-PKC beta 1 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKC beta 1 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC beta 1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PKC beta 1 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of K562 cells with PKC beta 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | PKC beta 1 [JM43-26] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BTN6 antibody BTNL11 antibody MGC26137 antibody MOG alpha 5 antibody MOG alpha 6 antibody MOG AluA antibody MOG AluB antibody MOG antibody MOG Ig AluB antibody MOG_HUMAN antibody MOGIG2 antibody Myelin oligodendrocyte glycoprotein antibody Myelin-oligodendrocyte glycoprotein antibody NRCLP7 antibody |
| Product description | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoformsandν. Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFC |
| WB:1:500~1:2000 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | BTN6 antibody BTNL11 antibody MGC26137 antibody MOG alpha 5 antibody MOG alpha 6 antibody MOG AluA antibody MOG AluB antibody MOG antibody MOG Ig AluB antibody MOG_HUMAN antibody MOGIG2 antibody Myelin oligodendrocyte glycoprotein antibody Myelin-oligodendrocyte glycoprotein antibody NRCLP7 antibody |
|---|---|
| Molecular Weight(MW) | 77 kDa |
| Cellular Localization | Cytoplasm. Nucleus. Membrane. |
Application
Fig1: Western blot analysis of PKC beta 1 on L929 cell using anti-PKC beta 1 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKC beta 1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC beta 1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PKC beta 1 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of K562 cells with PKC beta 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | L929, human tonsil tissue, human spleen tissue, mouse kidney tissue, K562. |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
