Application
Fig1: Western blot analysis of alpha Tubulin 4A on different cell lysate using anti-alpha Tubulin 4A antibody at 1/1,000 dilution. Positive control： Lane1: A431 Lane2: Rat brain tissue Lane3: NIH-3T3 Lane4: PC-12Application
Fig2: ICC staining alpha Tubulin 4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining alpha Tubulin 4A in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining alpha Tubulin 4A in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of Hela cells with alpha Tubulin 4A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | alpha Tubulin 4A [JM73-24] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha tubulin 1 antibody Alpha-tubulin 1 antibody FLJ30169 antibody H2 alpha antibody TBA4A_HUMAN antibody Testis specific alpha tubulin antibody Testis-specific alpha-tubulin antibody TUBA 4A antibody TUBA1 antibody Tuba4a antibody Tubulin alpha 1 (testis specific) antibody Tubulin alpha 1 antibody Tubulin alpha 1 chain antibody Tubulin alpha 4a antibody Tubulin alpha 4A chain antibody Tubulin alpha-1 chain antibody Tubulin alpha-4A chain antibody Tubulin H2 alpha antibody Tubulin H2-alpha antibody |
| Product description | Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:500-1:2,000 ICC:1:100-1:200 IHC:1:100-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Alpha tubulin 1 antibody Alpha-tubulin 1 antibody FLJ30169 antibody H2 alpha antibody TBA4A_HUMAN antibody Testis specific alpha tubulin antibody Testis-specific alpha-tubulin antibody TUBA 4A antibody TUBA1 antibody Tuba4a antibody Tubulin alpha 1 (testis specific) antibody Tubulin alpha 1 antibody Tubulin alpha 1 chain antibody Tubulin alpha 4a antibody Tubulin alpha 4A chain antibody Tubulin alpha-1 chain antibody Tubulin alpha-4A chain antibody Tubulin H2 alpha antibody Tubulin H2-alpha antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Cytoplasm, cytoskeleton. |
Application
Fig1: Western blot analysis of alpha Tubulin 4A on different cell lysate using anti-alpha Tubulin 4A antibody at 1/1,000 dilution. Positive control： Lane1: A431 Lane2: Rat brain tissue Lane3: NIH-3T3 Lane4: PC-12
Application
Fig2: ICC staining alpha Tubulin 4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining alpha Tubulin 4A in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining alpha Tubulin 4A in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of Hela cells with alpha Tubulin 4A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | WB:A431, NIH-3T3, PC-12, rat brain tissue;ICC: Hela, NIH-3T3, SW480; IHC: Human tonsil tissue, human thyroid tissue, mouse brain tissue, human placenta tissue, mouse testes tissue; FC: Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:200 IHC:1:100-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
