WB
Western blot analysis of Ephrin B2 on different lysates with Rabbit anti-Ephrin B2 antibody at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane), Lane 2: SK-OV-3 cell lysate (15 µg/Lane), Lane 3: Mouse lung tissue lysate (20 µg/Lane), Lane 4: Rat brain tissue lysate (20 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of C6 cells labeling Ephrin B2 with Rabbit anti-Ephrin B2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ephrin B2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | Ephrin B2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Ephrin-B and are conserved cytoplasmic tyrosine residues that are Eph receptors and ephrins optimistic supporting expression in many cases during embryogenesis and ephrin-B proteins may occur in expression domain interfaces. The transmembrane ligand ephrin-B2 and its receptor tyrosine Order a copy of this thesis Bidirectional signals mediated by both proteins play an important role in vascular development. Ephrin-B2 is essential for the normal morphogenesis of the embryonic vasculature and is angiogenic in tumors. It has been Identified as an important target of chemotheiples treatments. |
| Immunogen | Synthetic peptide within Human Ephrin B aa 47-96 / 333. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:2000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | EFN B2 antibody EFNB 2 antibody Efnb2 antibody EFNB2_HUMAN antibody Eph related receptor tyrosine kinase ligand 5 antibody EPH-related receptor tyrosine kinase ligand 5 antibody ephrin B2 antibody Ephrin-B2 antibody EphrinB2 antibody EPLG 5 antibody EPLG5 antibody Htk L antibody HTK ligand antibody HTK-L antibody HTKL antibody LERK 5 antibody LERK-5 antibody LERK5 antibody Ligand of eph related kinase 5 antibody MGC126226 antibody MGC126227 antibody MGC126228 antibody OTTMUSP00000024973 antibody |
|---|---|
| Molecular Weight(MW) | 37 kDa(Observed band size: 50 kDa) |
| Cellular Localization | Membrane. |
WB
Western blot analysis of Ephrin B2 on different lysates with Rabbit anti-Ephrin B2 antibody at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane), Lane 2: SK-OV-3 cell lysate (15 µg/Lane), Lane 3: Mouse lung tissue lysate (20 µg/Lane), Lane 4: Rat brain tissue lysate (20 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of C6 cells labeling Ephrin B2 with Rabbit anti-Ephrin B2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ephrin B2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:2000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
