Application
Fig1: Western blot analysis of IKB epsilon on different cell lysate using anti-IKB epsilon antibody at 1/1,000 dilution. Positive control： Line 1: THP-1 Line 2: Jurkat Line 3: HelaApplication
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IKB epsilon antibody. Counter stained with hematoxylin.| Product Name | IKB epsilon [JM62-63] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | I kappa B epsilon antibody I-kappa-B-epsilon antibody IkappaBepsilon antibody IkB E antibody IkB-E antibody IkB-epsilon antibody IKBE antibody IKBE_HUMAN antibody MGC72568 antibody NF kappa B inhibitor epsilon antibody NF kappa BIE antibody NF-kappa-B inhibitor epsilon antibody NF-kappa-BIE antibody NFkappa BIE antibody NFkappaB inhibitor epsilon antibody NFKBIE antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon antibody OTTHUMP00000016522 antibody Slc35b2 antibody solute carrier family 35, member B2 antibody |
| Product description | On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IκB family is represented by a protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IκB family member, IκB-ε, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500~1:2000 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | I kappa B epsilon antibody
I-kappa-B-epsilon antibody
IkappaBepsilon antibody
IkB E antibody
IkB-E antibody
IkB-epsilon antibody
IKBE antibody
IKBE_HUMAN antibody
MGC72568 antibody
NF kappa B inhibitor epsilon antibody
NF kappa BIE antibody
NF-kappa-B inhibitor epsilon antibody
NF-kappa-BIE antibody
NFkappa BIE antibody
NFkappaB inhibitor epsilon antibody
NFKBIE antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon antibody
OTTHUMP00000016522 antibody
Slc35b2 antibody
solute carrier family 35 member B2 antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | O00221 |
|---|
Application
Fig1: Western blot analysis of IKB epsilon on different cell lysate using anti-IKB epsilon antibody at 1/1,000 dilution. Positive control： Line 1: THP-1 Line 2: Jurkat Line 3: Hela
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IKB epsilon antibody. Counter stained with hematoxylin.| Positive Control | THP-1, Jurkat, Hela, human tonsil tissue. |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
