WB
Western blot analysis of GABARAP on different lysates with Rabbit anti-GABARAP antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: SH-SY5Y cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GABARAP antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GABARAP antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GABARAP antibody. Counter stained with hematoxylin.| Product Name | GABARAP Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | In the central nervous system GABA functions as the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing. GABA has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. In addition to GABA receptors, several proteins have been identified as regulators of GABA function, including GAD65, GAD67, GABA transporters and GABARAP (GABAA receptor-associated protein). GABARAP associates with GABAA Rg2 to link GABAA receptors to the cytoskeleton. The GABARAP protein sequence is similar to light chain-3 of microtubule-associated proteins (MAPs) suggesting that it may be a type of MAP or a component of a MAP complex. |
| Immunogen | Synthetic peptide within Human GABARAP aa 10-59 / 117. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | ATG8A antibody FLC 3B antibody FLC3B antibody FLJ25768 antibody GABA type A receptor associated protein antibody GABA(A) receptor associated protein antibody GABA(A) receptor-associated protein antibody GABARAP a antibody GABARAP antibody Gamma aminobutyric acid receptor associated protein antibody Gamma-aminobutyric acid receptor-associated protein antibody GBRAP_HUMAN antibody MGC120154 antibody MGC120155 antibody MM 46 antibody MM46 antibody |
|---|---|
| Molecular Weight(MW) | 14 kDa(Observed band size: 16 kDa) |
| Cellular Localization | Endomembrane system. Cytoplasm, cytoskeleton. |
WB
Western blot analysis of GABARAP on different lysates with Rabbit anti-GABARAP antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: SH-SY5Y cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GABARAP antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GABARAP antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GABARAP antibody. Counter stained with hematoxylin.| Application Notes | WB:1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
