Application
Fig1: ICC staining Ferritin Heavy Chain in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Ferritin Heavy Chain in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% TritonApplication
Fig3: ICC staining Ferritin Heavy Chain in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Ferritin Heavy Chain antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ferritin Heavy Chain antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Ferritin Heavy Chain antibody. Counter stained with hematoxylin.| Product Name | Ferritin Heavy Chain [JM22-36] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mammalian ferritins consist of 24 subunits made up of two types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of FeII, whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of FeIII. The most prominent role of mamma-lian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed; and oxidative stress, an important factor in the development of aging-related cataracts. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,IHC,ICC |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | Apoferritin antibody Cell proliferation inducing gene 15 protein antibody Cell proliferation-inducing gene 15 protein antibody F HC antibody Ferritin H subunit antibody Ferritin heavy chain antibody Ferritin heavy polypeptide 1 antibody FHC antibody FRIH antibody FRIH_HUMAN antibody FTH 1 antibody FTH antibody FTH1 antibody FTH1 protein antibody FTHL 6 antibody FTHL6 antibody Iron overload autosomal dominant antibody MGC104426 antibody N-terminally processed antibody OK/SW-cl.84 antibody PIG 15 antibody PIG15 antibody Placenta immunoregulatory factor antibody PLIF antibody Proliferation inducing gene 15 protein antibody Proliferation inducing protein 15 antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: ICC staining Ferritin Heavy Chain in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Ferritin Heavy Chain in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton
Application
Fig3: ICC staining Ferritin Heavy Chain in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Ferritin Heavy Chain antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ferritin Heavy Chain antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Ferritin Heavy Chain antibody. Counter stained with hematoxylin.| Positive Control | A549, Hela, PC-3M, human brain tissue, human kidney tissue, human placenta tissue. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
