Application
Fig1: Western blot analysis of SMYD3 on different cell/tissue lysate using anti-Profilin 1 antibody at 1/1,000 dilution. Positive control： Lane1: Rat kidney Lane2: PC-12 Lane3: Hela Lane4: Mouse spleen Lane5: NIH-3T3 Lane6: Rat spleenApplication
Fig2: ICC staining SMYD3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining SMYD3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining SMYD3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of Hela cells with SMYD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | SMYD3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | bA74P14.1 (novel protein) antibody bA74P14.1 antibody FLJ21080 antibody histone lysine N methyltransferase SMYD3 antibody KMT3E antibody MGC104324 antibody SET and MYND domain containing 3 antibody SET and MYND domain containing protein 3 antibody SET and MYND domain-containing protein 3 antibody SMYD 3 antibody Smyd3 antibody SMYD3 protein antibody SMYD3_HUMAN antibody Zinc finger MYND domain containing 1 antibody Zinc finger MYND domain containing protein 1 antibody Zinc finger MYND domain-containing protein 1 antibody Zinc finger protein subfamily 3A MYND domain containing 1 antibody Zinc finger protein, subfamily 3A (MYND domain containing), 1 antibody ZMYND 1 antibody ZMYND1 antibody ZNFN3A1 antibody |
| Product description | Belongs to the histone-lysine methyltransferase family. Contains 1 MYND-type zinc finger. Contains 1 SET domain. Histanes methyltransferase. Methylans 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC- 3 'or 5'-GAGGGG-3'. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:500-1:2,000 ICC/IF:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | bA74P14.1 (novel protein) antibody
bA74P14.1 antibody
FLJ21080 antibody
histone lysine N methyltransferase SMYD3 antibody
KMT3E antibody
MGC104324 antibody
SET and MYND domain containing 3 antibody
SET and MYND domain containing protein 3 antibody
SET and MYND domain-containing protein 3 antibody
SMYD 3 antibody
Smyd3 antibody
SMYD3 protein antibody
SMYD3_HUMAN antibody
Zinc finger MYND domain containing 1 antibody
Zinc finger MYND domain containing protein 1 antibody
Zinc finger MYND domain-containing protein 1 antibody
Zinc finger protein subfamily 3A MYND domain containing 1 antibody
Zinc finger protein subfamily 3A (MYND domain containing) 1 antibody ZMYND 1 antibody ZMYND1 antibody ZNFN3A1 antibody |
|---|---|
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of SMYD3 on different cell/tissue lysate using anti-Profilin 1 antibody at 1/1,000 dilution. Positive control： Lane1: Rat kidney Lane2: PC-12 Lane3: Hela Lane4: Mouse spleen Lane5: NIH-3T3 Lane6: Rat spleen
Application
Fig2: ICC staining SMYD3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining SMYD3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining SMYD3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of Hela cells with SMYD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | Rat kidney tissue lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, PC-12, NIH-3T3, HepG2, Hela, LOVO, human tonsil tissue, human colon cancer tissue, human placenta tissue, mouse stomach tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC/IF:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
