Application
Fig1: Western blot analysis of KPNA2 on different cell lysates using anti-KPNA2 antibody at 1/1,000 dilution. Postive control: Lane 1: Hela Lane 2: 293T Lane 3: HepG2 Lane 4: NIH-3T3 Lane 5: PC-12Application
Fig2: Flow cytometric analysis of 293T cells with KPNA2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | KPNA2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IMA1_HUMAN antibody Importin alpha 1 antibody Importin alpha 2 antibody Importin alpha 2 subunit antibody Importin alpha P1 antibody Importin subunit alpha-1 antibody IPO A1 antibody IPOA 1 antibody IPOA1 antibody Karyopherin alpha 2 antibody Karyopherin alpha 2 RAG cohort 1 antibody Karyopherin alpha 2 subunit antibody Karyopherin subunit alpha-2 antibody KPNA2 antibody KPNA2 protein antibody Pendulin antibody QIP 2 antibody QIP2 antibody RAG cohort 1 antibody RAG cohort protein 1 antibody RCH 1 antibody RCH1 antibody SRP 1 antibody SRP1 alpha antibody SRP1 antibody SRP1-alpha antibody SRP1alpha antibody |
| Product description | Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | IMA1_HUMAN antibody Importin alpha 1 antibody Importin alpha 2 antibody Importin alpha 2 subunit antibody Importin alpha P1 antibody Importin subunit alpha-1 antibody IPO A1 antibody IPOA 1 antibody IPOA1 antibody Karyopherin alpha 2 antibody Karyopherin alpha 2 RAG cohort 1 antibody Karyopherin alpha 2 subunit antibody Karyopherin subunit alpha-2 antibody KPNA2 antibody KPNA2 protein antibody Pendulin antibody QIP 2 antibody QIP2 antibody RAG cohort 1 antibody RAG cohort protein 1 antibody RCH 1 antibody RCH1 antibody SRP 1 antibody SRP1 alpha antibody SRP1 antibody SRP1-alpha antibody SRP1alpha antibody |
|---|---|
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of KPNA2 on different cell lysates using anti-KPNA2 antibody at 1/1,000 dilution. Postive control: Lane 1: Hela Lane 2: 293T Lane 3: HepG2 Lane 4: NIH-3T3 Lane 5: PC-12
Application
Fig2: Flow cytometric analysis of 293T cells with KPNA2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | HepG2, Hela, 293T, NIH-3T3, PC-12. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
