WB
Western blot analysis of mtTFA on different lysates with Rabbit anti-mtTFA antibody at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane), Lane 2: HL-60 cell lysate (20 µg/Lane), Lane 3: HeLa cell lysate (20 µg/Lane), Lane 4: HepG2 cell lysate (20 µg/Lane), Lane 5: MCF7 cell lysate (20 µg/Lane), Lane 6: Human brain tissue lysate (40 µg/Lane), Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-mtTFA antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling mtTFA with Rabbit anti-mtTFA antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-mtTFA antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.IF-P
Immunofluorescence analysis of paraffin-embedded human colon tissue labeling mtTFA with Rabbit anti-mtTFA antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).IP
mtTFA was immunoprecipitated from 0.2 mg K-562 cell lysate with Rabbit anti-mtTFA antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-mtTFA antibody at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody at 1/100,000 dilution was used for 1 hour at room temperature. Lane 1: K-562 cell lysate (input), Lane 2: Rabbit anti-mtTFA antibody IP in K-562 cell lysate, Lane 3: Rabbit K-562 instead of Rabbit anti-mtTFA antibody in 293T cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 1 minute.| Product Name | mtTFA Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M. |
| Immunogen | Synthetic peptide within Human mtTFA aa 41-90 / 246. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIF-PIHCIPWB |
| WB:1:1000 IHC:1:200-1:1000 ICC/IF:1:100-1:200 IF-P:1:50-1:200 IP:1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | anscription factor 6-like 1 antibody
Mitochondrial transcription factor 1 antibody
mitochondrial transcription factor A antibody
MtTF1 antibody
mtTFA antibody
TCF 6 antibody
TCF-6 antibody
TCF6 antibody
TCF6L1 antibody
TCF6L2 antibody
TCF6L3 antibody
TFAM antibody
TFAM_HUMAN antibody
Transcription factor 6 antibody
Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
Transcription factor 6 like 2 antibody
Transcription factor 6-like 2 antibody
transcription factor 6-like 3 antibody
Transcription factor A mitochondrial antibody Transcription factor A mitochondrial antibody Transcription factor A mitochondrial precursor antibody |
|---|---|
| Molecular Weight(MW) | 29 kDa(Observed band size: 24 kDa) |
| Cellular Localization | Mitochondrion, Mitochondrion matrix, mitochondrion nucleoid. |
| SwissProt ID | Q00059 |
|---|
WB
Western blot analysis of mtTFA on different lysates with Rabbit anti-mtTFA antibody at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane), Lane 2: HL-60 cell lysate (20 µg/Lane), Lane 3: HeLa cell lysate (20 µg/Lane), Lane 4: HepG2 cell lysate (20 µg/Lane), Lane 5: MCF7 cell lysate (20 µg/Lane), Lane 6: Human brain tissue lysate (40 µg/Lane), Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-mtTFA antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling mtTFA with Rabbit anti-mtTFA antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-mtTFA antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
IF-P
Immunofluorescence analysis of paraffin-embedded human colon tissue labeling mtTFA with Rabbit anti-mtTFA antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
IP
mtTFA was immunoprecipitated from 0.2 mg K-562 cell lysate with Rabbit anti-mtTFA antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-mtTFA antibody at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody at 1/100,000 dilution was used for 1 hour at room temperature. Lane 1: K-562 cell lysate (input), Lane 2: Rabbit anti-mtTFA antibody IP in K-562 cell lysate, Lane 3: Rabbit K-562 instead of Rabbit anti-mtTFA antibody in 293T cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 1 minute.| Application Notes | WB:1:1000 IHC:1:200-1:1000 ICC/IF:1:100-1:200 IF-P:1:50-1:200 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
