Application
Fig1: Western blot analysis of Cyclophilin F on human heart (1) and human brain (2) tissue lysate using anti-Cyclophilin F antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclophilin F antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Cyclophilin F antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Cyclophilin F antibody. Counter stained with hematoxylin.| Product Name | Cyclophilin F [JM71-39] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of cyclosporin A (CsA) and is encoded by a single unique gene conserved between yeast to humans. Cyclophilin B (CyPB) is secreted in biological fluids such as blood or milk, and binds to a specific receptor present on human peripheral blood lymphocytes and expressed in Jurkat cells, a line of human lymphoblasts. Cyclophilin D (CyP40) is a widely expressed cytoplasmic protein that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Cyclophilin F (CyP3), is also known as PPIF, Cyp-D or Rotamase F, is a 207 amino acid mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 20 kDa |
|---|---|
| Cellular Localization | Mitochondrion matrix. |
Application
Fig1: Western blot analysis of Cyclophilin F on human heart (1) and human brain (2) tissue lysate using anti-Cyclophilin F antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclophilin F antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Cyclophilin F antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Cyclophilin F antibody. Counter stained with hematoxylin.| Positive Control | Human heart tissue lysate, human brain tissue lysate, human colon cancer tissue, human breast cancer tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
