WB
Western blot analysis of SAE2 / UBA2 on different lysates with Rabbit anti-SAE2 / UBA2 antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate, Lane 2: A549 cell lysate, Lane 3: HT-29 cell lysate, Lane 4: K-562 cell lysate, Lane 5: RAW264.7 cell lysate, Lane 6: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 13 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-SAE2 / UBA2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-SAE2 / UBA2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | SAE2 / UBA2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The small ubiquitin-related modifier protein SUMO-1 belongs to the ubiquitin-like protein family, which are synthesized as precursor proteins that undergo processing before conjugation to target proteins. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processes, including nuclear transport, transcriptional regulation, apoptosis, and protein stability. SUMO-1 utilizes homologues of the E1 and E2 enzymes for conjugation to proteins, which include IkBα, MDM2, p53, PML, and RanGap1. AOS1 is homologous to the N-terminal half of E1 and UBA2 is homologous to the C-terminal half of E1. These proteins form a heterodimer that activates SUMO-1. |
| Immunogen | Synthetic peptide within Human SAE2 / UBA2 aa 39-88 / 640. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:5000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Anthracycline associated resistance ARX antibody Anthracycline-associated resistance ARX antibody ARX antibody FLJ13058 antibody HRIHFB2115 antibody SAE 2 antibody SAE2 antibody SAE2_HUMAN antibody SUMO 1 activating enzyme subunit 2 antibody SUMO activating enzyme subunit 2 antibody SUMO-activating enzyme subunit 2 antibody UBA2 antibody UBA2 ubiquitin activating enzyme E1 homolog antibody Ubiquitin like 1 activating enzyme E1B antibody Ubiquitin like modifier activating enzyme 2 antibody Ubiquitin-like 1-activating enzyme E1B antibody UBLE1B antibody |
|---|---|
| Molecular Weight(MW) | 71 kDa(Observed band size: 90 kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of SAE2 / UBA2 on different lysates with Rabbit anti-SAE2 / UBA2 antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate, Lane 2: A549 cell lysate, Lane 3: HT-29 cell lysate, Lane 4: K-562 cell lysate, Lane 5: RAW264.7 cell lysate, Lane 6: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 13 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-SAE2 / UBA2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-SAE2 / UBA2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:5000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
