Application
Fig1: Western blot analysis of DNA Ligase IV on Hela cell using anti-DNA Ligase IV antibody at 1/1,000 dilution. Positive control:Lane 1: HepG2Lane 2: HelaLane 3: JurkatApplication
Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-DNA Ligase IV antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DNA Ligase IV antibody. Counter stained with hematoxylin.| Product Name | DNA Ligase IV Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA. These stories form a critical complex composed of two molecules of each protein that preferentially bind DNA with nicks or broken ends. As an obligatory accessory molecule, XRCC4 binds to DNA Ligase IV and enhances its joining activity. The XRCC4 / DNA Ligase IV complex is also involved in V (D) J recombination. V (D) (in) a pair of high incidence of apoptosis in the development nervous system and a block in B and T cell maturation. |
| Immunogen | Recombinant protein within Human DNA Ligase IV aa 484-705 / 911. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICCIHCWB |
| WB:1:500~1:2000 IHC:1:50~1:200 ICC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | DNA ligase IV ATP dependent antibody DNA joinase antibody DNA ligase 4 antibody DNA ligase IV antibody DNA repair enzyme antibody DNLI4_HUMAN antibody LIG 4 antibody LIG4 antibody LIG4S antibody Ligase IV antibody Ligase IV DNA ATP dependent antibody Polydeoxyribonucleotide synthase [ATP] 4 antibody Polydeoxyribonucleotide synthase 4 antibody Polydeoxyribonucleotide synthase antibody Polynucleotide ligase antibody Sealase antibody |
|---|---|
| Molecular Weight(MW) | 104 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P49917 |
|---|
Application
Fig1: Western blot analysis of DNA Ligase IV on Hela cell using anti-DNA Ligase IV antibody at 1/1,000 dilution. Positive control:Lane 1: HepG2Lane 2: HelaLane 3: Jurkat
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-DNA Ligase IV antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DNA Ligase IV antibody. Counter stained with hematoxylin.| Positive Control | HepG2, Hela, Jurkat, human colon cancer tissue, human placenta tissue. |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 ICC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
