WB
Western blot analysis of Epac1 on different lysates with Rabbit anti-Epac1 antibody at 1/1,000 dilution. Lane 1: HUVEC cell lysate (20 µg/Lane), Lane 2: Mouse kidney tissue lysate (40 µg/Lane), Lane 3: Rat brain tissue lysate (40 µg/Lane), Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Epac1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Epac1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | 3',5' cyclic adenosine monophosphate (cAMP)-regulated guanine nucleotide exchange factors Epac (Epac1, cAMP-GEFI) and Epac2 (cAMP-GEFII) activate the Ras family GTPases Rap 1 and Rap 2 by promoting GTP binding in a cAMP-dependent manner. Eukaryotic cAMP is a second messenger that induces physiological responses such as gene expression, growth, differentiation, secretion and neurotransmission. The human Epac gene maps to chromosome 12q13.11 with transcript being abundant in the kidney and heart. In situ hybridization indicates expression of Epac in adult rat brain and selective expression in neonatal brain, including septum and thalamus. |
| Immunogen | Synthetic peptide within Human Epac1 aa 874-923 / 923. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:1,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RAPGEF3 |
|---|---|
| Gene Synonyms | EPAC EPAC1 bcm910 HSU79275 CAMP-GEFI |
| Gene Full Name | Rap guanine nucleotide exchange factor 3 |
| Alternative Names | bcm910 antibody CAMP GEFI antibody cAMP regulated guanine nucleotide exchange factor I antibody CAMPGEFI antibody CGEF 1 antibody CGEF1 antibody EPA1 antibody Epac 1 antibody EPAC antibody EPAC1 antibody Exchange factor directly activated by cAMP 1 antibody Exchange protein directly activated by cAMP 1 antibody MGC21410 antibody RAP guanine nucleotide exchange factor antibody Rap guanine nucleotide exchange factor (GEF) 3 antibody RAP guanine nucleotide exchange factor 3 antibody Rap1 guanine nucleotide exchange factor directly activated by cAMP antibody RAPGEF3 antibody |
| Molecular Weight(MW) | 95kDa(Predicted band size: 104kDa) |
| Cellular Localization | Endomembrane system. |
WB
Western blot analysis of Epac1 on different lysates with Rabbit anti-Epac1 antibody at 1/1,000 dilution. Lane 1: HUVEC cell lysate (20 µg/Lane), Lane 2: Mouse kidney tissue lysate (40 µg/Lane), Lane 3: Rat brain tissue lysate (40 µg/Lane), Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Epac1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Positive Control | Mouse kidney tissue lysate, human prostate tissue, human stomach cancer tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
