WB
Western blot analysis of EPAC1/RAPGEF3 using anti-EPAC1/RAPGEF3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Hela whole cell lysates, Lane 2: HepG2 whole cell lysates, Lane 3: COS7 whole cell lysates, Lane 4: T47D whole cell lysates, Lane 5: RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EPAC1/RAPGEF3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting SubstrateIHC
IHC analysis of EPAC1/RAPGEF3 using anti-EPAC1/RAPGEF3 antibody. EPAC1/RAPGEF3 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-EPAC1/RAPGEF3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of EPAC1/RAPGEF3 using anti-EPAC1/RAPGEF3 antibody. EPAC1/RAPGEF3 was detected in an immunocytochemical section of T-47D cells. The section was incubated with rabbit anti-EPAC1/RAPGEF3 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of MCF-7 cells using anti-EPAC1/RAPGEF3 antibody. Overlay histogram showing MCF-7 cells stained with anti-EPAC1/RAPGEF3 antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-EPAC1/RAPGEF3 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-EPAC1/RAPGEF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Epac1/RAPGEF3 recombinant protein (Position: H49-R881). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMonkeyMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAPGEF3 |
|---|---|
| Gene Synonyms | EPAC EPAC1 bcm910 HSU79275 CAMP-GEFI |
| Gene Full Name | Rap guanine nucleotide exchange factor 3 |
| Gene Summary | Enables guanyl-nucleotide exchange factor activity and protein domain specific binding activity. Involved in several processes, including Rap protein signal transduction; cellular response to cAMP; and regulation of syncytium formation by plasma membrane fusion. Located in several cellular components, including filopodium; lamellipodium; and microvillus. Implicated in nicotine dependence. Biomarker of congestive heart failure. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | CAMP GEFI CGEF1 EPAC EPAC 1 EPAC1 HSU79275 RAPGEF3 EPAC1 |
| Molecular Weight(MW) | 104kDa(Observed MW 104kDa) |
| Cellular Localization | membrane |
WB
Western blot analysis of EPAC1/RAPGEF3 using anti-EPAC1/RAPGEF3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Hela whole cell lysates, Lane 2: HepG2 whole cell lysates, Lane 3: COS7 whole cell lysates, Lane 4: T47D whole cell lysates, Lane 5: RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EPAC1/RAPGEF3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate
IHC
IHC analysis of EPAC1/RAPGEF3 using anti-EPAC1/RAPGEF3 antibody. EPAC1/RAPGEF3 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-EPAC1/RAPGEF3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of EPAC1/RAPGEF3 using anti-EPAC1/RAPGEF3 antibody. EPAC1/RAPGEF3 was detected in an immunocytochemical section of T-47D cells. The section was incubated with rabbit anti-EPAC1/RAPGEF3 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of MCF-7 cells using anti-EPAC1/RAPGEF3 antibody. Overlay histogram showing MCF-7 cells stained with anti-EPAC1/RAPGEF3 antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-EPAC1/RAPGEF3 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
