Application
Fig1: Western blot analysis of Cullin 1 on MCF-7 cell using anti-Cullin 1 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse oviduct tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with Cullin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | Cullin 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CUL 1 antibody CUL-1 antibody CUL1 antibody CUL1_HUMAN antibody Cullin-1 antibody Cullin1 antibody MGC149834 antibody MGC149835 antibody |
| Product description | Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIPFCICC/IF |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 ICC/IF:1:10-1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CUL 1 antibody CUL-1 antibody CUL1 antibody CUL1_HUMAN antibody Cullin-1 antibody Cullin1 antibody MGC149834 antibody MGC149835 antibody |
|---|---|
| Cellular Localization | Cytoplasm, Nucleoplasm. |
Application
Fig1: Western blot analysis of Cullin 1 on MCF-7 cell using anti-Cullin 1 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse oviduct tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with Cullin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | MCF-7, Hela, rat brain tissue, human stomach cancer tissue, mouse oviduct tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 ICC/IF:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
