ICC/IF
Phospho-Vinculin (Tyr1133/Tyr1065) Antibody staining Hela cells(4h of LPS treatment) by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Rabbit polyclonal antibody to Phospho-Vinculin (Tyr1133/Tyr1065) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and α-actinin. In addition, vinculin, Talin and α-actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. α-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin. |
| Immunogen | synthesized peptide derived from human Vinculin around the phosphorylation site of Tyr1133/1065. |
| Modification | p-Tyr1133/Tyr1065 |
| Modification Notes | Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques (By similarity). Acetylated; mainly by myristic acid but also by a small amount of palmitic acid. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| ICC/IF:1:100-1:500 |
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| Species Reactivity | HumanMouseRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | CMD1W antibody CMH15 antibody Epididymis luminal protein 114 antibody HEL114 antibody Metavinculin antibody MV antibody MVCL antibody OTTHUMP00000019861 antibody OTTHUMP00000019862 antibody VCL antibody VINC antibody VINC_HUMAN antibody Vinculin antibody |
|---|---|
| Molecular Weight(MW) | 124 kDa |
| Cellular Localization | Cytoplasm, cytoskeleton. |
ICC/IF
Phospho-Vinculin (Tyr1133/Tyr1065) Antibody staining Hela cells(4h of LPS treatment) by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | ICC/IF:1:100-1:500 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
