Application
Fig1: Western blot analysis of HPRT on different lysates using anti-HPRT antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: MCF-7 Lane 4: 293 Lane 5: Rat kidney Lane 6: Rat brain Lane 7: Mouse testis Lane 8: Mouse colonApplication
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.Application
Fig6: Western blot analysis of HPRT on Zebrafish tissue lysates using anti-HPRT antibody at 1/500 dilution.| Product Name | HPRT [JU03-26] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HGPRT antibody HGPRTase antibody HPRT 1 antibody HPRT_HUMAN antibody HPRT1 antibody Hypoxanthine guanine phosphoribosyltransferase antibody Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) antibody Hypoxanthine phosphoribosyltransferase 1 antibody Hypoxanthine-guanine phosphoribosyltransferase antibody |
| Product description | HPRT (hypoxanthine phosphoribosyltransferase 1), also known as HGPRT or HPRT1, is a 218 amino acid cytoplasmic protein that belongs to the purine/pyrimidine phosphoribosyltransferase family. Involved in purine metabolism, HPRT functions as a purine salvage enzyme that catalyzes the conversion of hypoxathine and guanine to their respective mononucleotides (inosine monophosphate and guanosine monophosphate, respectively). HPRT exists as a homotetramer that can bind two magnesium ions as cofactors. Defects in the gene encoding HPRT are the cause of gout and Lesch-Nyhan syndrome (LNS), both of which are characterized by a partial or complete lack of NPRT enzymatic activity. While a partial loss of HPRT enzymatic activity results in a buildup of uric acid (gout), a total loss of enzymatic activity results in hyperuricaemia, mental retardation, choreoathetosis and compulsive self-mutilation, all of which are symptoms associated with LNS. The severity of these diseases suggests an essential role for HPRT in purine metabolism. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500-1:2,000 IHC:1:50-1:200 IP:1:10-1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | HGPRT antibody HGPRTase antibody HPRT 1 antibody HPRT_HUMAN antibody HPRT1 antibody Hypoxanthine guanine phosphoribosyltransferase antibody Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) antibody Hypoxanthine phosphoribosyltransferase 1 antibody Hypoxanthine-guanine phosphoribosyltransferase antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of HPRT on different lysates using anti-HPRT antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: MCF-7 Lane 4: 293 Lane 5: Rat kidney Lane 6: Rat brain Lane 7: Mouse testis Lane 8: Mouse colon
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.
Application
Fig6: Western blot analysis of HPRT on Zebrafish tissue lysates using anti-HPRT antibody at 1/500 dilution.| Positive Control | Hela, A431, MCF-7, 293, rat kidney tissue lysate, rat brain tissue, mouse testis tissue lysate, mouse colon tissue lysate, Zebrafish tissue lysates, human tonsil tissue, human colon caner tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
