WB
Western blot analysis of USP10 on different lysates with Rabbit anti-USP10 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate, Lane 3: A549 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling USP10 with Rabbit anti-USP10 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-USP10 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.ICC/IF
ICC staining USP10 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.ICC/IF
ICC staining USP10 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-USP10 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human thyroid grand tissue using anti-USP10 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-USP10 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-USP10 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-USP10 antibody. Counter stained with hematoxylin.| Product Name | USP10 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP10 (ubiquitin specific peptidase 10), also known as UBPO, is a 798 amino acid protein that belongs to the ubiquitin-specific protease family of cysteine proteases. Expressed in a variety of tissues, USP10 functions to catalyze the cleavage of ubiquitin from ubiquitin-conjugated protein substrates, possibly playing a role in the activity of the DNA-bound androgen receptor complex. |
| Immunogen | Synthetic peptide within Human USP10 aa 28-77 / 798. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Deubiquitinating enzyme 10 antibody KIAA0190 antibody MGC124997 antibody MGC2621 antibody Ubiquitin carboxyl terminal hydrolase 10 antibody Ubiquitin carboxyl-terminal hydrolase 10 antibody Ubiquitin specific peptidase 10 antibody Ubiquitin specific processing protease 10 antibody Ubiquitin specific protease 10 antibody Ubiquitin thioesterase 10 antibody Ubiquitin thiolesterase 10 antibody Ubiquitin-specific-processing protease 10 antibody UBP10_HUMAN antibody UBPO antibody USP 10 antibody USP10 antibody |
|---|---|
| Molecular Weight(MW) | 87 kDa(Observed band size: 110 kDa) |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | Q14694 |
|---|
WB
Western blot analysis of USP10 on different lysates with Rabbit anti-USP10 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate, Lane 3: A549 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling USP10 with Rabbit anti-USP10 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-USP10 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
ICC/IF
ICC staining USP10 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC/IF
ICC staining USP10 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-USP10 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human thyroid grand tissue using anti-USP10 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-USP10 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-USP10 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-USP10 antibody. Counter stained with hematoxylin.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
