Application
Fig1: ICC staining Plexin A1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: Flow cytometric analysis of LOVO cells with Plexin A1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Plexin A1 [JU50-37] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOV antibody NOV/plexin A1 protein antibody NOVP antibody Plexin-A1 antibody Plexin1 antibody PlexinA1 antibody PLXA1_HUMAN antibody PLXN1 antibody Plxna1 antibody PLXNA1 protein antibody Semaphorin receptor NOV antibody |
| Product description | Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCFC |
| ICC:1:50-1:200 FC:1:50-1:100 WB:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | NOV antibody NOV/plexin A1 protein antibody NOVP antibody Plexin-A1 antibody Plexin1 antibody PlexinA1 antibody PLXA1_HUMAN antibody PLXN1 antibody Plxna1 antibody PLXNA1 protein antibody Semaphorin receptor NOV antibody |
|---|---|
| Molecular Weight(MW) | 211 kDa |
| Cellular Localization | Cell Membrane. |
Application
Fig1: ICC staining Plexin A1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: Flow cytometric analysis of LOVO cells with Plexin A1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | LOVO. |
|---|---|
| Application Notes | ICC:1:50-1:200 FC:1:50-1:100 WB:1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
