Application
Fig1: Western blot analysis of IKBKE on different cell lysates using anti-IKBKE antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: Raji Lane 3: MCF-7 Lane 4: JurkatApplication
Fig2: ICC staining IKBKE in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining IKBKE in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining IKBKE in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IKBKE antibody. Counter stained with hematoxylin.Application
Application| Product Name | IKBKE [JU06-72] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | I kappa B kinase epsilon antibody I-kappa-B kinase epsilon antibody IkBKE antibody IKK related kinase epsilon antibody IKK-E antibody IKK-epsilon antibody IKK-i antibody IKKE antibody IKKE_HUMAN antibody IKKepsilon antibody IKKI antibody Inducible I kappa B kinase antibody Inducible I kappa-B kinase antibody Inducible IkappaB kinase antibody Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon antibody Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody KIAA0151 antibody MGC125294 antibody MGC125295 antibody MGC125297 antibody |
| Product description | The transcription factor NFκB is retained in the cytoplasm in an inactive form by the inhibitory protein IκB. Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκB-α and specifically phosphorylates IκB-α on the sites that trigger its degradation, Serines 32 and 36. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IκB phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKKα and IKKβ. IKK-i is primarily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNFα, IL-1 and IL-6. Overexpression of IKK-i has been shown to result in phosphorylation of IκBα on Ser 32 and Ser 36, and in NFκB activation, suggesting that IKK-i may act as an IκB kinase in the immune system. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCFCIHC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | I kappa B kinase epsilon antibody
I-kappa-B kinase epsilon antibody
IkBKE antibody
IKK related kinase epsilon antibody
IKK-E antibody
IKK-epsilon antibody
IKK-i antibody
IKKE antibody
IKKE_HUMAN antibody
IKKepsilon antibody
IKKI antibody
Inducible I kappa B kinase antibody
Inducible I kappa-B kinase antibody
Inducible IkappaB kinase antibody
Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody
Inhibitor of kappa light polypeptide gene enhancer in B cells kinase of epsilon antibody Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody KIAA0151 antibody MGC125294 antibody MGC125295 antibody MGC125297 antibody |
|---|---|
| Molecular Weight(MW) | 80 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | Q14164 |
|---|
Application
Fig1: Western blot analysis of IKBKE on different cell lysates using anti-IKBKE antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: Raji Lane 3: MCF-7 Lane 4: Jurkat
Application
Fig2: ICC staining IKBKE in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining IKBKE in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining IKBKE in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IKBKE antibody. Counter stained with hematoxylin.
Application
Application| Positive Control | Hela, Raji, MCF-7, Jurkat, JAR, SK-Br-3, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
