Application
Fig1: Western blot analysis of alpha Internexin on different lysates using anti-alpha Internexin antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain tissue Lane 2: A549Application
Fig2: ICC staining alpha Internexin in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-alpha Internexin antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-alpha Internexin antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of M2A cells with alpha Internexin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | alpha Internexin [JU25-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 66 kDa neurofilament protein antibody AINX_HUMAN antibody Alpha Inx antibody Alpha-internexin antibody Alpha-Inx antibody INA antibody Internexin neuronal intermediate filament protein alpha antibody MGC12702 antibody NEF 5 antibody NEF5 antibody Neurofilament 5 (66kD) antibody Neurofilament 5 antibody Neurofilament 66 antibody Neurofilament 66 tax binding protein antibody Neurofilament-66 antibody NF 66 antibody NF-66 antibody NF66 antibody TXBP 1 antibody TXBP1 antibody |
| Product description | Alpha-internexin is a brain specific type IV intermediate filament protein. This axonal protein is found in most, if not all, neurons of the CNS. The head domain of alpha-internexin is essential for self-assembly into a filament network. Expression levels of alpha-internexin have been shown to be maximal during late embryogenesis and to decline into adulthood, suggesting that this protein plays a role in regulatory processes during the development of the brain. The alpha-internexin promoter has been shown to be activated by Brn-3a or Brn-3c transcription factor binding, while Brn-3b binding to the promoter results in alpha-internexin repression. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFCIP |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 66 kDa neurofilament protein antibody AINX_HUMAN antibody Alpha Inx antibody Alpha-internexin antibody Alpha-Inx antibody INA antibody Internexin neuronal intermediate filament protein alpha antibody MGC12702 antibody NEF 5 antibody NEF5 antibody Neurofilament 5 (66kD) antibody Neurofilament 5 antibody Neurofilament 66 antibody Neurofilament 66 tax binding protein antibody Neurofilament-66 antibody NF 66 antibody NF-66 antibody NF66 antibody TXBP 1 antibody TXBP1 antibody |
|---|---|
| Molecular Weight(MW) | 66 kDa |
| Cellular Localization | Nucleus, cytoskeleton, extracellular space. |
Application
Fig1: Western blot analysis of alpha Internexin on different lysates using anti-alpha Internexin antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain tissue Lane 2: A549
Application
Fig2: ICC staining alpha Internexin in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-alpha Internexin antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-alpha Internexin antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of M2A cells with alpha Internexin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Mouse brain tissue lysate, A549, PC-12, rat brain tissue, mouse cerebellum tissue, N2A. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
