WB
Western blot analysis of Transglutaminase 2 on different lysates with Rabbit anti-Transglutaminase 2 antibody at 1/1,000 dilution. Lane 1: K-562 cell lysate, Lane 2: HeLa cell lysate, Lane 3: HUVEC cell lysate, Lane 4: Mouse placenta tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 12 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-Transglutaminase 2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat placenta tissue with Rabbit anti-Transglutaminase 2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Transglutaminase 2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Terminally differentiating mammalian epidermal cells acquire an insoluble, 10 to 20 nm thick protein deposit on the intracellular surface of the plasma membrane known as the cross-linked cell envelope (CE). The CE is a component of the epidermis that is generated through formation of disulfide bonds and g-glutamyl-lysine isodipeptide bonds, which are formed by the action of transglutaminases (TGases). TGases are intercellularly localizing, Ca2+-dependent enzymes that catalyze the formation of isopeptide bonds by transferring an amine on to glutaminyl residues, thereby cross-linking glutamine residues and lysine residues in substrate proteins. TGases influence numerous biological processes, including blood coagulation, epidermal differentiation, seminal fluid coagulation, fertilization, cell differentiation and apoptosis. Human keratinocyte transglutaminase (TGase1) is a membrane associated, 817 amino acid protein. Human tissue transglutaminase (TGase2) is an endothelial cell specific, 687 amino acid protein. |
| Immunogen | Synthetic peptide within Human Transglutaminase 2 aa 578-627 / 687. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | ALPHA SUBUNIT antibody C polypeptide antibody EC 2.3.2.13 antibodyepididymis secretory protein Li 45 antibody G alpha h antibody G[a]h antibody Gh CLASS G ALPHA h antibody GNAH antibody GNAH G PROTEIN antibody H POLYPEPTIDE antibody HEL-S-45 antibody Protein glutamine gamma glutamyltransferase 2 antibody Protein-glutamine gamma-glutamyltransferase 2 antibody TG 2 antibody TG(C) antibody TG2 antibody TGase C antibody TGase H antibody TGase-2 antibody TgaseII antibody TGC antibody TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody TGM2 antibody TGM2_HUMAN antibody Tissue transglutaminase antibody Transglutaminase 2 antibody Transglutaminase 2 C polypeptide antibody Transglutaminase C antibody Transglutaminase H antibody Transglutaminase-2 antibody tTG antibody tTGas antibody |
|---|---|
| Molecular Weight(MW) | 77 kDa(Observed band size: 70-77 kDa) |
| Cellular Localization | Cytosol. Plasma Membrane. |
WB
Western blot analysis of Transglutaminase 2 on different lysates with Rabbit anti-Transglutaminase 2 antibody at 1/1,000 dilution. Lane 1: K-562 cell lysate, Lane 2: HeLa cell lysate, Lane 3: HUVEC cell lysate, Lane 4: Mouse placenta tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 12 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-Transglutaminase 2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat placenta tissue with Rabbit anti-Transglutaminase 2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:2000 IHC:1:200 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
