Application
Fig5: Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig5: Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig5: Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig5: Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig5: Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Mad2L2 [JU99-23] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Homolog of REV7 S cerevisiae antibody hREV7 antibody MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody MAD2 homolog antibody MAD2 like 2 antibody MAD2 mitotic arrest deficient like 2 antibody MAD2-like protein 2 antibody MAD2B antibody Mad2l2 antibody MD2L2_HUMAN antibody Mitotic Arrest Deficient 2 L2 antibody Mitotic arrest deficient 2-like protein 2 antibody Mitotic arrest deficient homolog like 2 antibody Mitotic arrest deficient like 2 (yeast) antibody Mitotic arrest deficient yeast homolog antibody Mitotic spindle assembly checkpoint protein MAD2B antibody Polymerase (DNA directed) zeta 2 accessory subunit antibody POLZ2 antibody REV 7 antibody REV7 antibody REV7 homolog antibody Weakly similar to Mitotic MAD2 protein (S cerevisiae) antibody |
| Product description | Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T-47D, was shown to complete mitosis in the presence of Nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during pro-metaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with Insulin receptor (IR), but not IGF-IR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and, if the process is not complete, MAD2B delays the onset of anaphase. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFCIP |
| WB:1:500 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Homolog of REV7 S cerevisiae antibody
hREV7 antibody
MAD2 (mitotic arrest deficient yeast homolog) like 2 antibody MAD2 homolog antibody MAD2 like 2 antibody MAD2 mitotic arrest deficient like 2 antibody MAD2-like protein 2 antibody MAD2B antibody Mad2l2 antibody MD2L2_HUMAN antibody Mitotic Arrest Deficient 2 L2 antibody Mitotic arrest deficient 2-like protein 2 antibody Mitotic arrest deficient homolog like 2 antibody Mitotic arrest deficient like 2 (yeast) antibody Mitotic arrest deficient yeast homolog antibody Mitotic spindle assembly checkpoint protein MAD2B antibody Polymerase (DNA directed) zeta 2 accessory subunit antibody POLZ2 antibody REV 7 antibody REV7 antibody REV7 homolog antibody Weakly similar to Mitotic MAD2 protein (S cerevisiae) antibody |
|---|---|
| Molecular Weight(MW) | 24 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
Application
Fig5: Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig5: Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig5: Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig5: Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig5: Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | SiHa, rat lung tissue, mouse colon tissue, human tonsil tissue, Hela. |
|---|---|
| Application Notes | WB:1:500 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
