Application
Fig1: Western blot analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution. Positive control: Lane 1: NIH-3T3 Lane 2: Hela Lane 3: Rat brain Lane 4: Mouse testisApplication
Fig2: ICC staining RbAP48 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining RbAP48 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining RbAP48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of Hela cells with RbAP48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | RbAP48 [JU37-47] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAF I p48 antibody CAF-1 subunit C antibody CAF-I 48 kDa subunit antibody CAF-I p48 antibody Chromatin assembly factor 1 subunit C antibody Chromatin assembly factor I p48 subunit antibody Chromatin assembly factor/CAF 1 p48 subunit antibody Histone-binding protein RBBP4 antibody MSI1 protein homolog antibody Nucleosome-remodeling factor subunit RBAP48 antibody NURF55 antibody RB binding protein 4 chromatin remodeling factor antibody RbAp 48 antibody RBAP48 antibody RBBP-4 antibody RBBP4 antibody RBBP4_HUMAN antibody Retinoblastoma binding protein 4 antibody Retinoblastoma binding protein p48 antibody Retinoblastoma-binding protein 4 antibody Retinoblastoma-binding protein p48 antibody |
| Product description | In the intact cell, DNA is closely associated with histones and other nuclear proteins to form chromatin.The remodeling of chromatin is believed to be a critical component of transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation, and an increased accessiblity of DNA to transcription factors. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. The retinoblastoma binding proteins RbAp46 and RbAp48 have been identified as histone binding proteins, and they are components of the histone deacetylase complex. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCFCIP |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CAF I p48 antibody CAF-1 subunit C antibody CAF-I 48 kDa subunit antibody CAF-I p48 antibody Chromatin assembly factor 1 subunit C antibody Chromatin assembly factor I p48 subunit antibody Chromatin assembly factor/CAF 1 p48 subunit antibody Histone-binding protein RBBP4 antibody MSI1 protein homolog antibody Nucleosome-remodeling factor subunit RBAP48 antibody NURF55 antibody RB binding protein 4 chromatin remodeling factor antibody RbAp 48 antibody RBAP48 antibody RBBP-4 antibody RBBP4 antibody RBBP4_HUMAN antibody Retinoblastoma binding protein 4 antibody Retinoblastoma binding protein p48 antibody Retinoblastoma-binding protein 4 antibody Retinoblastoma-binding protein p48 antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution. Positive control: Lane 1: NIH-3T3 Lane 2: Hela Lane 3: Rat brain Lane 4: Mouse testis
Application
Fig2: ICC staining RbAP48 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining RbAP48 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining RbAP48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of Hela cells with RbAP48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | NIH-3T3, Hela, rat brain tissue, mouse testis tissue, MCF-7, SH-SY5Y, human tonsil tissue, human lung cancer tissue, human liver tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
