Application
Fig1: Western blot analysis of EAAT3 on different lysates with Rabbit anti-EAAT3 antibody at 1/2,000 dilution. Lane 1: Human brain tissue lysate, Lane 2: Human lung tissue lysate, Lysates/proteins at 40 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.Application
Fig2: Flow cytometric analysis of SH-SY5Y cells labeling EAAT3. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig3: ICC staining EAAT3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining EAAT3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.| Product Name | EAAT3 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Can also transport L-cysteine. Functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion. Mediates Cl- flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na+ symport. Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate. Negatively regulated by ARL6IP5. |
| Immunogen | Recombinant protein within Human EAAT3 aa 80-209 / 524. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000 ICC/IF:1:50-1:200 IHC:1:50-1:200 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | EAA3_HUMAN antibody
EAAC 1 antibody
EAAC1 antibody
EAAT 3 antibody
Excitatory amino acid carrier 1 antibody
Excitatory amino acid carrier1 antibody
Excitatory amino acid transporter 3 antibody
Excitatory amino acid transporter3 antibody
Excitatory amino-acid carrier 1 antibody
GLUTAMATE TRANSPORTER HIGH-AFFINITY antibody MEAAC 1 antibody MEAAC1 antibody Neuronal and epithelial glutamate transporter antibody REAAC 1 antibody REAAC1 antibody Slc1 a1 antibody Slc1a 1 antibody SLC1A1 antibody Sodium dependent glutamate/aspartate transporter 3 antibody Sodium-dependent glutamate/aspartate transporter 3 antibody Solute carrier family 1 (neuronal / epithelial high affinity glutamate transporter system Xag) member 1 antibody SOLUTE CARRIER FAMILY 1 (NEURONAL/EPITHELIAL HIGH AFFINITY GLUTAMATE TRANSPORTER) MEMBER 1 antibody Solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter system Xag) member 1 antibody Solute carrier family 1 member 1 antibody Solute carrier family 1 member 1 antibody |
|---|---|
| Molecular Weight(MW) | 57kDa(Observed band size: 60kDa) |
| Cellular Localization | Cell membrane. |
Application
Fig1: Western blot analysis of EAAT3 on different lysates with Rabbit anti-EAAT3 antibody at 1/2,000 dilution. Lane 1: Human brain tissue lysate, Lane 2: Human lung tissue lysate, Lysates/proteins at 40 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Flow cytometric analysis of SH-SY5Y cells labeling EAAT3. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig3: ICC staining EAAT3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining EAAT3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.| Positive Control | Mouse liver tissue lysate, HUVEC, LOVO, SH-SY5Y, rat brain tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue.Cell membrane. |
|---|---|
| Application Notes | WB:1:2000 ICC/IF:1:50-1:200 IHC:1:50-1:200 FC:1:1000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
