WB
Western blot analysis of IGFBP2 on different lysates with Rabbit anti-IGFBP2 antibody at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate, Lane 2: T-47D cell lysate, Lane 3: MCF7 cell lysate, Lane 4: BxPC-3 cell lysate, Lane 5: JEG-3 cell lysate (low expression), Lane 6: COS-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma tissue with Rabbit anti-IGFBP2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IGFBP2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | IGFBP2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The Insulin-like growth factor-binding proteins (IGFBPs), a family of homologous proteins that have co-evolved with the IGFs, serve not only as shuttle molecules for the soluble IGFs, but also confer a level of regulation to the IGF signaling system. Physical association of the IGFBPs with IGF influences the bio-availability of the growth factors, and their concentration and distribution in the extracellular environment. The IGFBPs also appear to have biological activity independent of the IGFs. Seven IGFBPs have been described, each differing in their tissue distribution, half-lives and modulation of IGF interactions with their receptors. IGFBP1 is negatively regulated by Insulin production. The IGFBP1 gene is expressed at a high level during fetal liver development and in response to nutritional changes and diabetes. IGFBP2, which may function as a chaperone, escorting IGFs to their target tissues, is expressed in several human tissues including fetal eye and fetal brain. |
| Immunogen | Synthetic peptide within C-terminal Human IGFBP2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:5000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | BP 2 antibody BP2 antibody IBP 2 antibody IBP-2 antibody IBP2 antibody IBP2_HUMAN antibody IGF binding protein 2 antibody IGF BP53 antibody IGF-binding protein 2 antibody IGFBP 2 antibody IGFBP-2 antibody IGFBP2 antibody IGFBP53 antibody Insulin like growth factor binding protein 2 36kDa antibody Insulin like growth factor binding protein 2 antibody Insulin like growth factor-binding protein 2 precursor antibody Insulin-like growth factor-binding protein 2 antibody |
|---|---|
| Molecular Weight(MW) | 35 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P18065 |
|---|
WB
Western blot analysis of IGFBP2 on different lysates with Rabbit anti-IGFBP2 antibody at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate, Lane 2: T-47D cell lysate, Lane 3: MCF7 cell lysate, Lane 4: BxPC-3 cell lysate, Lane 5: JEG-3 cell lysate (low expression), Lane 6: COS-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma tissue with Rabbit anti-IGFBP2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IGFBP2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Positive Control | Human serum lysate, NCCIT, human liver tissue. |
|---|---|
| Application Notes | WB:1:5000 IHC:1:200-1:1000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
