WB
Western blot analysis of ALDH1L1 on different lysates with Rabbit anti-ALDH1L1 antibody at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate, Lane 2: Mouse liver tissue lysate, Lane 3: Mouse kidney tissue lysate, Lane 4: Rat brain tissue lysate, Lane 5: Rat liver tissue lysate, Lane 6: Rat kidney tissue lysate, Lysates/proteins at 40 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ALDH1L1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-ALDH1L1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-ALDH1L1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Species: Mouse, Site: Cerebral cortex, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Not required.IF-F
Species: Rat, Site: Cerebral cortex, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Not required.IF-P
Species: Mouse, Site: Cerebral cortex, Sample: Paraffin-embedded section, Antibody concentration: 1:500.IF-P
Species: Rat, Site: Cerebral cortex, Sample: Paraffin-embedded section, Antibody concentration: 1:500.| Product Name | ALDH1L1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | 10-formyltetrahydrofolate dehydrogenase is an enzyme that in humans is encoded by the ALDH1L1 gene. The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning. |
| Immunogen | Recombinant protein within Human ALDH1L1 aa 155-375 . |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IF-FIF-PIHCWB |
| WB:1:5000-1:10000 IHC:1:500-1:2000 IF-F:1:500-1:1000 IF-P:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | 10 formyltetrahydrofolate dehydrogenase antibody 10 FTHFDH antibody 10-formyltetrahydrofolate dehydrogenase antibody 10-FTHFDH antibody AL1L1_HUMAN antibody Aldehyde dehydrogenase 1 family member L1 antibody Aldehyde dehydrogenase family 1 member L1 antibody aldh1l1 antibody Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody DKFZp781N0997 antibody EC 1.5.1.6 antibody FDH antibody formyltetrahydrofolate dehydrogenase antibody FTHFD antibody OTTHUMP00000155221 antibody OTTHUMP00000214696 antibody OTTHUMP00000214700 antibody OTTHUMP00000222724 antibody |
|---|---|
| Molecular Weight(MW) | 99 kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of ALDH1L1 on different lysates with Rabbit anti-ALDH1L1 antibody at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate, Lane 2: Mouse liver tissue lysate, Lane 3: Mouse kidney tissue lysate, Lane 4: Rat brain tissue lysate, Lane 5: Rat liver tissue lysate, Lane 6: Rat kidney tissue lysate, Lysates/proteins at 40 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ALDH1L1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-ALDH1L1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-ALDH1L1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Species: Mouse, Site: Cerebral cortex, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Not required.
IF-F
Species: Rat, Site: Cerebral cortex, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Not required.
IF-P
Species: Mouse, Site: Cerebral cortex, Sample: Paraffin-embedded section, Antibody concentration: 1:500.
IF-P
Species: Rat, Site: Cerebral cortex, Sample: Paraffin-embedded section, Antibody concentration: 1:500.| Application Notes | WB:1:5000-1:10000 IHC:1:500-1:2000 IF-F:1:500-1:1000 IF-P:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
