WB
Western blot analysis of SCGB1A1 on different lysates with Rabbit anti-SCGB1A1 antibody at 1/1,000 dilution. Lane 1: MCF7 (Human breast cancer cell) cell lysate, Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate, Lane 3: C6 (Rat glioma cell) cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 180 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/1,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.ICC/IF
ICC staining of SCGB1A1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).ICC/IF
ICC staining of SCGB1A1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).FC
Flow cytometric analysis of SCGB1A1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | SCGB1A1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SCGB1A1 also named CC10. Clara cell 10 (CC10) protein, a homologue of rabbit uteroglobin, is a phospholipase A2 inhibitor. CC10 is regulated by AP-1, octamer, and hepatocyte nuclear factor-3 (HNF-3) family transcription factors. CC10 expression changes in relation to the ovarian menstrual cycle, and expression in human endometrium may be stimulated by progesterone, suggesting that CC10 may regulate eicosanoid levels in the human uterus. CC10 is expressed in nonciliated airway epithelial cells in the lung and in urogenital secretions. CC10 is involved in modulating inflammation in airway passages and may play a role in asthma. Overexpression of CC10 in the non-small cell lung cancer cell line A549 was shown to result in the near absence of MMP-2 and MMP-9 matrix metalloproteinases and a reduction in invasiveness, indicating that loss of CC10 may contribute to carcinogenesis. |
| Immunogen | Synthetic peptide within Human SCGB1A1 aa 42-91 / 91. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Blastokinin antibody
CC10 antibody
CC16 antibody
CCPBP antibody
CCSP antibody
Clara cell phospholipid binding protein antibody
Clara cell phospholipid-binding protein antibody
Clara cell specific 10 kD protein antibody
Clara cells 10 kDa secretory protein antibody
OTTHUMP00000236107 antibody
SCGB1A1 antibody
Secretoglobin family 1A member 1 antibody
Secretoglobin family 1A member 1 (uteroglobin) antibody UG antibody UGB antibody UP-1 antibody UP1 antibody Urinary protein 1 antibody Urine protein 1 antibody UTER_HUMAN antibody Uteroglobin antibody |
|---|---|
| Molecular Weight(MW) | 10 kDa(Observed band size: 15 kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of SCGB1A1 on different lysates with Rabbit anti-SCGB1A1 antibody at 1/1,000 dilution. Lane 1: MCF7 (Human breast cancer cell) cell lysate, Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate, Lane 3: C6 (Rat glioma cell) cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 180 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/1,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
ICC/IF
ICC staining of SCGB1A1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC/IF
ICC staining of SCGB1A1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
FC
Flow cytometric analysis of SCGB1A1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
