Application
Fig1: Western blot analysis of Coilin on rat kidney tissue lysates using anti-Coilin at 1/500 dilution.Application
Fig2: Flow cytometric analysis of Jurkat cells with Coilin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Coilin [JU44-08] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLN 80 antibody CLN80 antibody COIL antibody COIL_HUMAN antibody Coilin antibody Coilin p80 antibody p80 antibody P80 coilin antibody |
| Product description | Coilin protein is one of the main molecular components of Cajal bodies (CBs). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. In addition to its structural role, coilin acts as glue to connect the CB to the nucleolus. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome and chromosome. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CLN 80 antibody CLN80 antibody COIL antibody COIL_HUMAN antibody Coilin antibody Coilin p80 antibody p80 antibody P80 coilin antibody |
|---|---|
| Molecular Weight(MW) | 63 kDa (Predicted band size) |
| Cellular Localization | Nucleus. |
| SwissProt ID | P38432 |
|---|
Application
Fig1: Western blot analysis of Coilin on rat kidney tissue lysates using anti-Coilin at 1/500 dilution.
Application
Fig2: Flow cytometric analysis of Jurkat cells with Coilin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Rat kidney tissue lysates, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
