WB
Western blot analysis of MAPKAP Kinase 2 on SK-Br-3 cell lysate using anti-MAPKAP Kinase 2 antibody at 1/500 dilution.WB
Western blot analysis of MAPKAP Kinase 2 on different lysates with Rabbit anti-MAPKAP Kinase 2 antibody at 1/2,000 dilution. Lane 1: HCT 116-si NT cell lysate, Lane 2: HCT 116-si MAPKAP Kinase 2 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | MAPKAP Kinase 2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The MAPKAP kinases (for MAP kinase activated protein kinases) are a group of MAP kinase substrates which are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identifed and serve as substrates for the ERK and p38 MAP kinases. These include the serine/threonine kinases Rsk-1 (also designated MAPKAP kinase-1), Rsk-2 and Rsk-3, which are phosphorylated by ERK1 and ERK2. Similarly, p38 phosphorylates and activates the serine/threonine kinases MAPKAP kinase-2 and MAPKAP kinase-3 (also designated 3pK). The serine/threonine kinases Mnk1 and Mnk2 are substrates for both ERK and p38 MAP kinases. |
| Immunogen | Synthetic peptide within Human MAPKAP Kinase 2 aa 351-400 / 400. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:500-1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | MAP kinase activated protein Kinase 2 antibody MAP kinase-activated protein kinase 2 antibody MAPK activated protein kinase 2 antibody MAPK-activated protein kinase 2 antibody MAPK2_HUMAN antibody MAPKAP K2 antibody MAPKAP kinase 2 antibody MAPKAPK 2 antibody MAPKAPK-2 antibody MAPKAPK2 antibody Mitogen activated protein kinase activated protein kinase 2 antibody MK 2 antibody MK2 antibody |
|---|---|
| Molecular Weight(MW) | 46 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Western blot analysis of MAPKAP Kinase 2 on SK-Br-3 cell lysate using anti-MAPKAP Kinase 2 antibody at 1/500 dilution.
WB
Western blot analysis of MAPKAP Kinase 2 on different lysates with Rabbit anti-MAPKAP Kinase 2 antibody at 1/2,000 dilution. Lane 1: HCT 116-si NT cell lysate, Lane 2: HCT 116-si MAPKAP Kinase 2 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
